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Buffer Detection Kit for Magnetic Beads
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506291
Sigma-AldrichLactimidomycin - CAS 134869-15-1 - Calbiochem
This product can no longer be ordered through emdmillipore.com. Please refer to (Cat. No. 506291) on sigmaaldrich.com.
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MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
A glutarimide based 12-membered macrolide antibiotic with antifungal properties. Acts as a potent inhibitor of cell migration, cell proliferation, and protein synthesis (IC50 = 37.82 nM), but does not affect RNA synthesis even at high concentrations (IC50 > 10 µM). Acts as a potent inhibitor of translation and appears to block the first translocation step without affecting tRNA binding or peptide bond formation. It exhibits about 10-fold greater potency than cycloheximide (Cat. No. 239763 and 239764). Binds to the same site on the 60S ribosomal subunit with high affinity (Kd = 500 nM vs 15 µM for cycloheximide). Shown to extend the survival of mice bearing P388 lymphoma by blocking cell growth, however, it does not significantly affect non-tumorigenic MCF10A breast cell lines. Also shown to retard the growth of MDA MB231 cells in nude mice (~0.6 /mg/kg).
Catalogue Number
506291
Brand Family
Calbiochem®
Synonyms
LTM
References
References
Schneider-Poetsch, T., et al. 2010. Nat. Chem. Biol.6, 209. Micoine, K., et al. 2010. J. Am. Chem. Soc.132, 14064. Ju, J., et al. 2009. J. Am. Chem. Soc.131, 1370. Sugawara, K., et al. 1992. J. Antibiot.45, 1433.
Schneider-Poetsch, T., et al. 2010. Nat. Chem. Biol.6, 209. Micoine, K., et al. 2010. J. Am. Chem. Soc.132, 14064. Ju, J., et al. 2009. J. Am. Chem. Soc.131, 1370. Sugawara, K., et al. 1992. J. Antibiot.45, 1433.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
20-September-2013 JSW
Synonyms
LTM
Description
A glutarimide based 12-membered macrolide antibiotic with antifungal properties. Acts as a potent inhibitor of cell migration, cell proliferation, and protein synthesis (IC50 = 37.82 nM), but does not affect RNA synthesis even at high concentrations (IC50 > 10 µM). Acts as a potent inhibitor of translation and appears to block the first translocation step without affecting tRNA binding or peptide bond formation. It exhibits about 10-fold greater potency than cycloheximide (Cat. No. 239763 and 239764). Binds to the same site on the 60S ribosomal subunit with high affinity (Kd = 500 nM vs 15 µM for cycloheximide). Shown to extend the survival of mice bearing P388 lymphoma by blocking cell growth, however, it does not significantly affect non-tumorigenic MCF10A breast cell lines. Also shown to retard the growth of MDA MB231 cells in nude mice (~0.6 /mg/kg).
Form
White powder
Intert gas (Yes/No)
Packaged under inert gas
CAS number
134869-15-1
Chemical formula
C₂₆H₃₅NO₆
Structure formula
Purity
≥98% by HPLC
Solubility
DMSO
Storage
Protect from light -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Schneider-Poetsch, T., et al. 2010. Nat. Chem. Biol.6, 209. Micoine, K., et al. 2010. J. Am. Chem. Soc.132, 14064. Ju, J., et al. 2009. J. Am. Chem. Soc.131, 1370. Sugawara, K., et al. 1992. J. Antibiot.45, 1433.
