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23-028M IkBa Protein, activated, 250 µg

Activated, full length, recombinant human IκBα. N-terminal c-Myc, 6His-tagged. Phosphorylated in vitro using IKKα, active. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.

Key Applications: Enzyme Assays
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23-028M
250 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsSpeciesCascade Component
      Enzyme AssaysHuman Substrate
      Description
      Catalogue Number23-028M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionIkBa Protein, activated, 250 µg
      OverviewMillipore’s Universal Transportation Medium (UTM) is room temperature, sterile, stable medium which can sustain viability (and infectivity) of a pleurality of organisms that include clinically important viruses, chlamidyiae, mycoplasmas, or ureaplasmas during transit to the testing laboratory. UTM is provided with labeled capture-cap vials designed for transport of a sample. The capture cap is designed to secure the shaft of the swab sample to the cap, eliminating the use of forceps to remove the swab in the laboratory.

      The formulation consists of modified Hank’s balanced salt solution supplemented with bovine serum albumin, cysteine, gelatin, sucrose and glutamic acid. The pH is buffered with HEPES buffer. Phenol red is used to indicate pH. Vancomycin, amphotericin B, and colistin are incorporated in the medium to inhibit growth of competing bacteria and yeast.
      Alternate Names
      • NF-kappa-B inhibitor alpha
      • I-kappa-B-alpha
      • Major histocompatibility complex enhancer-binding protein MAD3
      Background InformationIκBα is a cellular protein that inhibits the transcription factor NF-κB. NF-κB plays a central role in cellular stress and inflammatory responses by controlling cytokine-inducible gene expression and lymphocyte stimulation by antigens. In non-stimulated cells NF-κB is sequestered in the cytoplasm via the inhibitory protein IκBα, which obscures the nuclear localization signal of NF-κB and also blocks its ability to bind DNA. In response to various stimuli, including viral infection, ultraviolet radiation and inflammatory cytokines, IκBα is phosphorylated by the protein kinase complex IKK, which triggers its rapid degradation by ubiquitin mediated proteolysis. The liberated NF-κB then translocates to the nucleus and activates the expression of target genes. Mutations in the gene for IκBα result in the functional impairment of NF-κB which causes a decreased production of proinflammatory cytokines and certain interferons, rendering patients susceptible to infection.
      References
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationActivated, full length, recombinant human IκBα. N-terminal c-Myc, 6His-tagged. Phosphorylated in vitro using IKKα, active. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.
      Key Applications
      • Enzyme Assays
      Biological Information
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Cascade ComponentSubstrate
      Entrez Gene Number
      Purification MethodPurified using immobilized metal affinity chromatography.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      23-028M 04053252294440

      Documentation

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      23-028 IkBa Protein, activated, 10 µg Show Pricing & Availability

      Product Families

      Ubiquitin Proteasome System: Enzymes and Substrates

      Millipore has removed the complexity of Ubiquitination by providing functionally validated enzymes and substrates, making the pathway readily accessible for research and drug discovery.
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      Categories

      Life Science Research > Drug Discovery and Development > Ubiquitin and Proteasome Screening > Ubiquitination Substrates
      Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Ubiquitination Substrates