71012 Sigma-AldrichHT96™ BL21(DE3) Competent Cells - Novagen
The HT96 BL21(DE3) Competent Cells are designed for high-throughput transformation and protein expression applications.
More>> The HT96 BL21(DE3) Competent Cells are designed for high-throughput transformation and protein expression applications. Less<<HT96™ BL21(DE3) Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview
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Pricing & Availability
| Catalogue Number | Availability | Packaging | Qty/Pack | Price | Quantity | |
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| 71012-3 |
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Glass bottle | 1 ea |
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| 71012-4 |
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Glass bottle | 4 ea |
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| Description | |
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| Overview | The HT96™ BL21(DE3) Competent Cells are
designed for high-throughput transformation.
The cells are predispensed in 20-µl volumes
in a sturdy 96-well polypropylene plate,
which is compatible with a variety of thermal
cyclers and water baths. Wells are individually
sealed and have raised rims to prevent cross-contamination.
Seals can be easily pierced
with standard pipet tips or removed for access.
Strips of caps are also provided for reliable
sealing during manipulation and storage.
Groups of 24 wells can be easily split from
the plate for processing smaller numbers of
samples.
HT96 BL21(DE3) Competent Cells BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases. The designation (DE3) indicates that the strain is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. |
| Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com. |
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| Catalogue Number | 71012 |
| Brand Family | Novagen® |
| Features and benefits | • High-throughput, 96-well format |
| References | |
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| References | Phillips, T.A., et al. 1984. J. Bacteriol. 159, 283. |
| Product Information | |
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| Components | |
| Guaranteed efficiency | > 2 × 10⁶ cfu/µg |
| Quality Level | MQ100 |
| Applications |
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| Biological Information |
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| Physicochemical Information |
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| Dimensions |
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| Materials Information |
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| Toxicological Information |
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| Safety Information according to GHS |
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| Safety Information |
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| Product Usage Statements |
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| Storage and Shipping Information | |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Do not freeze | Ok to freeze |
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| Transport Information |
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| Supplemental Information |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 71012-3 | 04055977255737 |
| 71012-4 | 04055977255744 |
Documentation
HT96™ BL21(DE3) Competent Cells - Novagen SDS
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HT96™ BL21(DE3) Competent Cells - Novagen Certificates of Analysis
| Title | Lot Number |
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| 71012 |
References
| Reference overview |
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| Phillips, T.A., et al. 1984. J. Bacteriol. 159, 283. |
User Protocols
| Title |
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| TB055 pET System Manual |
| TB313 HT96™ Competent Cells |