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109217 Gram's safranine solution

for the Gram staining method

Gram's safranine solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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109217
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Overview

Replacement Information

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      		 Plastic bottle 500 ml
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      1092172500
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          		 Plastic bottle 2.5 l
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          1092179025
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              		 Barrel PE/met 25 l
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              Description
              Catalogue Number109217
              DescriptionGram's safranine solution
              OverviewThis ready-to-use Gram's safranine solution-for the Gram staining method, is used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution (Cat. no.109218) - an aniline dye - in the first step. After the treatment with iodinesolution (Lugol’s solution stabilized with PVP, Cat no.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear pink to red.
              A bottle with 500 ml Gram's safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Cat. no.1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
              References
              Product Information
              HS Code3212 90 90
              Quality LevelMQ400
              Applications
              Applicationfor the Gram staining method
              Biological Information
              Physicochemical Information
              Density0.98 g/cm3 (20 °C)
              Flash point49 °C
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Hazard Pictogram(s) Hazard Pictogram(s)
              Hazard Statement(s)H226: Flammable liquid and vapour.
              Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
              P233: Keep container tightly closed.
              P240: Ground and bond container and receiving equipment.
              P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
              P242: Use non-sparking tools.
              P243: Take action to prevent static discharges.
              Signal WordWarning
              Storage class3 Flammable liquids
              WGKWGK 1 slightly hazardous to water
              Disposal28
              Aqueous solutions: Container D.
              Safety Information
              Categories of dangerflammable
              Product Usage Statements
              Storage and Shipping Information
              StorageStore at +15°C to +25°C.
              Packaging Information
              Transport Information
              Declaration (railroad and road) ADR, RIDKein Gefahrgut
              Declaration (transport by air) IATA-DGRNo Dangerous Good
              Declaration (transport by sea) IMDG-CodeNo Dangerous Good
              Supplemental Information
              Specifications
              Suitability for microscopypasses test
              gram-positve microorganismsblue-violet
              gram-negative microorganismspink to red
              Global Trade Item Number
              Catalogue Number GTIN
              1092170500 04022536112046
              1092172500 04022536557397
              1092179025 04022536641003

              Related Products & Applications

              Alternative Products

              Catalogue Number Description  
              111885 Gram-Color Show Pricing & Availability

              Related Products

              Catalogue Number Description  
              111885 Gram-Color Show Pricing & Availability

              Accessories (Primary)

              Catalogue Number Description  
              100567 Lugol's solution Show Pricing & Availability
              109218 Gram's crystal violet solution Show Pricing & Availability
              109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability
              110218 Gram's decolorizing solution Show Pricing & Availability

              Accessories (Secondary)

              Catalogue Number Description  
              100567 Lugol's solution Show Pricing & Availability
              109218 Gram's crystal violet solution Show Pricing & Availability
              109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability
              111885 Gram-Color Show Pricing & Availability

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              Categories

              IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining