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SCC116 GT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line

GT1-7 cells can be used as an in vitro model of GnRH-secreting neurons of the hypothalamus.

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Key Applications: Cell Culture, Cell Based Assays
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SCC116
≥1X10⁶ cells/vial  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      CULT, Cell Based Assays
      Description
      Catalogue NumberSCC116
      DescriptionGT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line
      OverviewGT1-7 is an immortalized mature mouse hypothalamic GnRH neuronal cell line. Immortalized GnRH neurons were generated by introducing a transgene containing the promotor region of the GnRH gene coupled to the coding region of the SV40 T-antigen oncogene into transgenic mice1. The resulting anterior hypothalamic tumors were removed from one of the mice and the cells dissociated and cloned. GT1-7 is a clonal line of mature differentiated GnRH neurons that exhibit high levels of Gnrh1 mRNA and secrete GnRH in response to depolarization.
      Alternate Names
      • GT1-7 hypothalamic cell line
      • GT1-7 neural cell line
      Background InformationGonadotrophin-releasing hormone (GnRH) is a neuropeptide that is synthesized and released from GnRH neurons within the hypothalamus and is required for normal reproductive development and function. The scarcity and scattered distribution of GnRH neurons in the rostral hypothalamus make the biological study of the cells difficult.

      References:
      1. Mellon PL, et.al. (1990) Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis. Neuron 5(1): 1-10.
      2. Liposits Z, et.al. (1991) Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone. Endocrinology 129(3): 1575-1583.
      3. Wetsel WC, et.al. (1991) Metabolism of pro-luteinizing hormone-releasing hormone in immortalized hypothalamic neurons. Endocrinology 129(3): 1584-1595.
      4. Wetsel WC, et.al. (1992) Intrinsic pulsatile secretory activity of immortalized luteinizing hormone-releasing hormone-secreting neurons Proc. Natl. Acad. Sci. USA 89(9): 4149-4153.
      5. Whyte DB, et.al. (1995) A neuron-specific enhancer targets expression of the gonadotropin-releasing hormone gene to hypothalamic neurosecretory neurons. Mol. Endocrinol 9(4): 467-477.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationGT1-7 cells can be used as an in vitro model of GnRH-secreting neurons of the hypothalamus.
      Key Applications
      • Cell Culture
      • Cell Based Assays
      Application NotesThis product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Neural Lineage Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
      • Cells tested negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC116 04054839165849