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345762 GDF-8/Myostatin, Human, Mouse, Rat, Recombinant

345762
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Overview

Key Spec Table

Purity
≥80% by SDS-PAGE

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345762-10UG
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      Description
      OverviewRecombinant, human mouse rat growth differentiation factor 8 (GDF-8) consisting of amino acids 268-376 and expressed in NS0-derived murine myeloma cells. GDF-8 is expressed in developing and adult skeletal muscle and is 100% identical to mature human, rat, mouse, and cow at the amino acid sequence level. Useful for the inhibition of preadipocyte differentiation to adipocytes.
      Catalogue Number345762
      Brand Family Calbiochem®
      SynonymsMyostatin
      References
      ReferencesKim, H.S., et al. 2001. Biochem. Biophys. Res. Commun. 281, 902.
      Lee, S.J., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 9306.
      Sharma, M., et al. 1999. J. Cell Physiol. 180, 1.
      McPherron, A.C., et al. 1997. Nature 387, 83.
      Storm, E.E., et al. 1994. Nature 368, 639.
      Product Information
      FormSolid
      FormulationLyophilized from 0.2 µm filtered Acetonitrile, TFA, BSA.
      Quality LevelMQ100
      Applications
      ApplicationGDF-8/Myostatin, Human, Mouse, Rat, Recombinant, consists of amino acids 268-376 and is expressed in NS0-derived murine myeloma cells. Useful for inhibiting preadipocyte differentiation to adipocytes.
      Biological Information
      Biological activityHemoglobin Expression:
      ED₅₀ = 2-10 ng/ml as measured by induction of hemoglobin expression in k562 human chronic myelogenous leukemia cells.

      Elongation of Animal Caps:
      Optimal Elongation of animal caps from Xenpus blastulae.
      Purity≥80% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: <1.0 EU per/µg protein
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Global Trade Item Number
      Catalogue Number GTIN
      345762-10UG 04055977193954