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324726 Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli

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324726
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324726-200MIU
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      Description
      OverviewRecombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. This enzyme is not active above pH 6.0.
      Note: 1 mU = 1 milliunit.
      Catalogue Number324726
      Brand Family Calbiochem®
      SynonymsEndo-β-N-acetylglucosaminidase F2, Endo F2
      References
      ReferencesReddy, A., et al. 1998. Glycobiology 8, 633.
      Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
      Product Information
      Activity≥5 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5.
      EC number3.2.1.96
      FormLiquid
      FormulationIn 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5.
      Quality LevelMQ100
      Biological Information
      Specific Activity≥20 units/mg protein
      Physicochemical Information
      ContaminantsN-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      324726-200MIU 04055977216004