324726 Sigma-AldrichEndoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli
Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides. It is not active above pH 6.0.
More>> Endoglycosidase F2, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides. It is not active above pH 6.0. Less<<Synonyms: Endo-β-N-acetylglucosaminidase F2, Endo F2
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Pricing & Availability
Catalogue Number | Availability | Packaging | Qty/Pack | Price | Quantity | |
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324726-200MIU |
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Plastic ampoule | 200 miu |
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Description | |
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Overview | Recombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and biantennary complex oligosaccharides from glycoproteins. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. This enzyme is not active above pH 6.0. |
Note: 1 mU = 1 milliunit. | |
Catalogue Number | 324726 |
Brand Family | Calbiochem® |
Synonyms | Endo-β-N-acetylglucosaminidase F2, Endo F2 |
Product Information | |
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Activity | ≥5 units/ml |
Unit of Definition | One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1 µmol porcine fibrinogen per min at 37°C, pH 4.5. |
EC number | 3.2.1.96 |
Form | Liquid |
Formulation | In 25 mM NaCl, 10 mM sodium acetate buffer, pH 4.5. |
Quality Level | MQ100 |
Biological Information | |
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Specific Activity | ≥20 units/mg protein |
Physicochemical Information | |
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Contaminants | N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected |
Storage and Shipping Information | |
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Ship Code | Blue Ice Only |
Toxicity | Standard Handling |
Storage | +2°C to +8°C |
Do not freeze | Yes |
Global Trade Item Number | |
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Catalogue Number | GTIN |
324726-200MIU | 04055977216004 |