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S8005 CpGenome™ 5-mC and 5-hmC DNA Standard Set

S8005
2 µg per vial  
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      Overview

      Description
      Catalogue NumberS8005
      Brand Family Upstate
      Trade Name
      • CpGenome
      • Upstate
      DescriptionCpGenome™ 5-mC and 5-hmC DNA Standard Set
      OverviewCpGenome™ 5-mC and 5-hmC DNA Standard Set contains three linear, double-stranded DNA (897 bp) with the same sequence, but each contains 100 % 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines. This set can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications.

      Materials Provided:

      Three vials, each containing 2 µg (40 µL) of CpGenome™ Cytosine DNA Standard, CpGenome™ 5-mC DNA Standard, or CpGenome™ 5-hmC DNA Standard, at a concentration of 50 ng/μL.

      Validation:

      The three Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.

      CpGenome is a trademark of Serologicals Corporation.
      Product Information
      PresentationLiquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
      Quality LevelMQ100
      Applications
      ApplicationThis set can be used to calibrate applications aimed at analyzing & quantifying cytosine modifications.
      Biological Information
      Species Reactivity
      • Human
      Modifications
      • Methylation
      • Hydroxylation
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended Storage: Stable for 6 months at -20°C from date of receipt.
      Packaging Information
      Material Size2 µg per vial
      Global Trade Item Number
      Catalogue Number GTIN
      S8005 04053252648427