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SCT025 BioTracker 519 Green β-Gal Dye

Live cell imaging dye for β-galactosidase (β-gal) used to detect LacZ reporter gene expression and cellular senescence (SA-β-gal).

BioTracker 519 Green β-Gal Dye MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Based Assays
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SCT025
5x30nmol  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      Cell Based Assays
      Description
      Catalogue NumberSCT025
      DescriptionBioTracker 519 Green β-Gal Dye
      Overviewβ-galactosidase, also called beta-gal or β-gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond. β-gal is commonly used in molecular biology as a reporter marker to monitor gene expression using the chromogenic X-Gal. β-gal has also been used to measure cellular senescence (SA-β-gal).

      The BioTracker 519 Green β-Gal Dye is a fluorescent probe for the detection of β-galactosidase in living cells. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence. Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements. The dye can stain LacZ expressing HEK293 cells both before and after 3% paraformaldehyde fixation.

      Spectral Properties
      Absorbance: 497nm
      Emission: 519nm
      Alternate Names
      • GlycoGREEN™–βGal
      References
      Product Information
      Detection methodFluorescence
      PresentationLyophilized
      Applications
      ApplicationLive cell imaging dye for β-galactosidase (β-gal) used to detect LacZ reporter gene expression and cellular senescence (SA-β-gal).
      Key Applications
      • Cell Based Assays
      Application NotesLive cell fluorescent imaging
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker 519 Green β-Gal Dye at -20°C, desiccate and protect from light
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening
      Packaging Information
      Material Size5x30nmol
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT025 04054839545580