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SCCAR10109-1VL Assay Ready AC16 Human Cardiomyocyte Cell Line

AC16 Human Cardiomyocytes can differentiate into cardiomyocytes when cultured in mitogen-free medium. The cells may be used to study developmental biology of cardiomyocytes and cardiovascular disease. **SCCAR10109 cells are non-expandable and packaged at high density to enable readiness for hepatic fibrosis and other relevant assays without propagation or passage.

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      Overview

      Replacement Information
      Description
      Catalogue NumberSCCAR10109-1VL
      DescriptionAssay Ready AC16 Human Cardiomyocyte Cell Line
      OverviewThis cell line may not be expanded. It is provided at a cell density that provides sufficient cells for a 96-well plate assay upon recovery.
      Alternate Names
      • AC16 Ventricular Cardiomyocyte Cell Line
      Background InformationThis product contains cells sold under a consumable label license and which may not be propagated. For the equivalent expandable, bankable LX-2 cell line, please see SCC109.

      AC16 Human Cardiomyocyte Cell Line is a proliferating cell line that was derived from the fusion of primary cells from adult human ventricular heart tissues with SV40 transformed, uridine auxotroph human fibroblasts, devoid of mitochondrial DNA (1, 2). After selection in uridine-free medium to remove unfused fibroblasts, the resulting fused cells were further subcloned and subsequently screened for the presence of SV40 large T-ag, β-myosin heavy chain (βMHC) and connexin-43 (CX-43) (1).

      AC16 cells are proliferating and differentiation of the cells may be controlled by altering culture conditions and by silencing the expression of SV40 T-Ag (1). The cells can be used to study cardiac gene expression and function, during normal development and in pathological conditions at the cellular, organellar and molecular levels.

      References:
      1. Davidson, M. M., Nesti, C., Palenzuela, L., Walker, W. F., Hernandez, E., Protas, L., Hirano, M., Isaac, N. D. (2005) Novel cell lines derived from adult human ventricular cardiomyocytes. J. Mol. Cell Cardiol. 39(1): 133-147.
      2. Litzkas, P., Jha, K.K., Ozer, H.L. (1984) Efficient transfer of cloned DNA into human diploid cells: protoplast fusion in suspension. Mol. Cell. Biol 4(11): 2549-2552.
      References
      Product Information
      Components
      • 1 vial AC16 human cardiomyocyte cells (Catalog No. SCCAR10109-1VL). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationAC16 Human Cardiomyocytes can differentiate into cardiomyocytes when cultured in mitogen-free medium. The cells may be used to study developmental biology of cardiomyocytes and cardiovascular disease. **SCCAR10109 cells are non-expandable and packaged at high density to enable readiness for hepatic fibrosis and other relevant assays without propagation or passage.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale as a consumable. It may not be propagated, passaged, or expanded and is subject to the terms of the “Assay Ready Cells Research Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
      Biological Information
      Cell Line Type
      • Cardiomyocyte Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
      • Cells are negative for mycoplasma contamination.
      • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsAC16 Human Cardiomyocyte Cell Line should be stored in liquid nitrogen.
      Packaging Information
      Material Size1 vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCCAR10109-1VL 04065271953505