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ABE2888-50UG Anti-phospho PPAR alpha (Ser73)

Anti-phospho PPARalpha (Ser73), Cat. No. ABE2888, is a highly specific rabbit polyclonal antibody that targets Peroxisome proliferator-activated receptor alpha (PPARalpha) and has been tested in Dot Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, and Western Blotting.

Anti-phospho PPAR alpha (Ser73) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: PPARA, NR1C1, PPAR UniProt Number: Q07869
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ABE2888-50UG
50 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MDB, ICC, IHC, IF, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2888-50UG
      DescriptionAnti-phospho PPAR alpha (Ser73)
      Alternate Names
      • Peroxisome proliferator-activated receptor alpha
      • PPAR-alpha
      • Nuclear receptor subfamily 1 group C member 1
      Background InformationPeroxisome proliferator-activated receptor alpha (UniProt: Q07869; also known as PPAR-alpha, Nuclear receptor subfamily 1 group C member 1) is encoded by the PPARA (also known as NR1C1, PPAR) gene (Gene ID: 5465) in human. PPARs are members of the nuclear hormone receptor family of transcription factors that mediate a variety of cellular processes, including glucose and lipid metabolism, inflammatory responses, and regulation of apoptotic cell death. They act by binding to specific peroxisome proliferator-response elements (PPREs) on target genes. Three forms of PPARs have been described, which are designated as alpha, beta, and delta forms. PPAR alpha contains a DNA binding domain (aa 99-173) and a ligand-binding domain. It also has two NR C-4 type of zinc finger regions (aa 102-122 and 139-161) that bind to the regulator region of DNA when the receptor is activated. The ligand-binding domain has an extensive secondary structure of several alpha- helices and a beta-sheets. PPAR alpha heterodimerizes with RXRA and this heterodimerization is required for it DNA binding and transactivation activity. Two isoforms of PPAR alpha have been described that are produced by alternative splicing. PPAR alpha is expressed in skeletal muscle, liver, kidney, and endothelial cells and regulates lipoprotein metabolism. Its transcriptional activity is enhanced in the presence of insulin. Leukotriene B4 (LTB4) is shown to bind to PPAR alpha and induce transcription of genes of the omega and beta-oxidation pathways and clofibrate that binds to PPAR alpha accelerates catabolism of LTB4 in granulocytes and macrophages. PPARalpha-deficient mice show a prolonged inflammatory response when exposed to LTB4. GSK3-beta is reported to phosphorylate serine 73 on PPAR alpha, which in turn increases ubiquitination and protein turnover and reduces its activity.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.1% sodium azide.
      Applications
      ApplicationAnti-phospho PPARalpha (Ser73), Cat. No. ABE2888, is a highly specific rabbit polyclonal antibody that targets Peroxisome proliferator-activated receptor alpha (PPARalpha) and has been tested in Dot Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Dot Blot
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis): A 1:250 dilution from a representative lot detected phospho PPAR alpha (Ser73) in lentiviral infected 3T3-L1 cells with vector or PPARalpha overexpressed (Courtesy of Dr. Terry Hinds at University of Toledo).

      Immunofluorescence Analysis: A representative lot detected phospho PPAR alpha (Ser73) in Immunofluorescence applications (Hinds, T.D., et. al. (2016). J Biol Chem. 291(48):25179-25191).

      Immunocytochemistry Analysis: A representative lot detected phospho PPAR alpha (Ser73) in Immunocytochemistry applications (Hinds, T.D., et. al. (2016). J Biol Chem. 291(48):25179-25191).

      Immunohistochemistry Analysis: A representative lot detected phospho PPAR alpha (Ser73) in Immunohistochemistry applications (Hinds, T.D., et. al. (2017). Am J Physiol Endocrinol Metab. 312(4):E244-E252).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids surrounding phospho serine 73 of human Peroxisome proliferator-activated receptor alpha (PPAR alpha).
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Peroxisome proliferator-activated receptor alpha (PPAR alpha) phosphorylated on serine 73.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Rat, Bovine, Human based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PPARA
      • NR1C1
      • PPAR
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~52 kDa observed; 52.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Bot in phospho PPAR (Ser73) peptide.



      Dot Blot Analysis: A 1:250 dilution from a representative lot detected phospho PPAR (Ser73) peptide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2888-50UG 04054839550850