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ABC467 Anti-phospho BAD Antibody (Ser155)

Anti-phospho BAD (Ser155) is an antibody against phospho BAD (Ser155) for use in WB, PIA, IP.

Anti-phospho BAD Antibody (Ser155) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: BAD, BBC6, BCL2L8 UniProt Number: Q92934
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ABC467
200 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIA, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC467
      DescriptionAnti-phospho BAD Antibody (Ser155)
      Alternate Names
      • Bcl2 antagonist of cell death
      • BAD
      • Bcl-2-binding component 6
      • Bcl-2-like protein 8
      • Bcl2-L-8
      • Bcl-XL/Bcl-2-associated death promoter
      Background InformationBad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118, Ser-134, and Ser-155 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho BAD (Ser155) is an antibody against phospho BAD (Ser155) for use in WB, PIA, IP.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      • Immunoprecipitation
      Application NotesPeptide Inhibition Analysis: A 1:100 dilution from a representative lot blocked phospho BAD (Ser155) in Forskolin treated PC12 cell lysates.
      Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated phospho BAD (Ser155) in 0.5 mg of Forskolin treated PC12 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the BH3 domain of human phospho BAD (Ser155).
      EpitopeBH3 domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Bovine, Canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BAD
      • BBC6
      • BCL2L8
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~20 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Forskolin treated PC12 cell lysate.

      Western Blotting Analysis: A 1:250 dilution of this antibody detected phospho BAD (Ser155) in 10 µg of Forskolin treated PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC467 04055977113563

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies