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MAB8151-I-100UL Anti-West Nile Virus/Kunjin Antibody, Envelope Antibody, clone 3.91D

Anti-West Nile Virus/Kunjin, Envelope, clone 3.91D, Cat. No. MAB8151-I, is a mouse monoclonal antibody that detects envelope protein in West Nile virus and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

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MAB8151-I-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB8151-I-100UL
      DescriptionAnti-West Nile Virus/Kunjin Antibody, Envelope Antibody, clone 3.91D
      Alternate Names
      • Envelope protein
      • WNV Envelope Protein
      Background InformationGenome polyprotein (UniProt: P14335) is encoded by the POLY gene (Gene ID: 912267) in West Nile virus. West Nile virus (WNV) is a mosquito-borne positive-sense RNA virus of the Flavivirus genus within the Flaviviridae family. WNV genome encodes for three structural domains known as C, prM, and E and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). These are translated as a single polypeptide, which is subsequently cleaved by viral and cellular proteases. The envelope glycoprotein (E) undergoes a conformational rearrangement triggered by low pH that results in a class II fusion event required for viral entry. The E protein (aa 291-791) contains a three-domain structure where domain I is an eight-stranded β-barrel in the center portion of the protein (aa 1-51, 134-195, and 284-297). The DII domain consists of 170 residues inserted into DI (aa 52-133 and 196-283). At the distal end of DII domain is a fusion loop (aa 98-110) and a conserved glycine-rich, hydrophobic sequence, which is important for flaviviral fusion. The third domain, DIII, is an Ig-like domain with seven β-strand and has been implicated in receptor binding. The E protein also has a single N-linked glycosylation site at Asn 154, located within the ectodomain of prM domain and is cleaved by cellular enzymes during viral particle maturation. Clone E60 specifically binds to the DII fusion loop and displays limited neutralizing activity and even at higher doses neutralization activity is shown to be between 26 and 68%. (Ref.: Martín-Acebes MA, et al. (2012). World J Virol. 1(2); 51-70).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-West Nile Virus/Kunjin, Envelope, clone 3.91D, Cat. No. MAB8151-I, is a mouse monoclonal antibody that detects envelope protein in West Nile virus and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • ELISA
      Application NotesWestern Blotting Analysis: A representative lot detected West Nile Virus/Kunjin, Envelope protein in Western Blotting applications (Maeda, A., et al. (2009). Virus Res.;144(1-2):35-43; Saiyasombat, R., et al. (2014). Virol J.;11:150; Blitvich, B.J., et al. (2016). Am J Trop Med Hyg.;95(5):1185-1191).

      ELISA Analysis: Various dilutions of this antibody detected recombinant West Nile Virus/Kunjin, Envelope protein.

      Immunofluorescence Analysis: A representative lot detected West Nile Virus/Kunjin, Envelope protein in Immunofluorescence applications (Maeda, A., et al. (2009). Virus Res.;144(1-2):35-43; Osorio, J.E., et al. (2012). Am J Trop Med Hyg.;87(3):565-72).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenWest Nile Virus (WNV; subtype KUNV) isolate OR393.
      EpitopeN-terminal half
      Clone3.91D
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3.91D is a mouse monoclonal antibody that detects West Nile virus envelope protein. It targets an epitope within the N-terminal half of polyprotein.
      IsotypeIgG3
      Species Reactivity
      • Virus
      Species Reactivity NoteWest Nile virus.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • E
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~50 kDa observed; 31.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant West Nile Virus envelope protein.

      Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant West Nile Virus envelope protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB8151-I-100UL 04065266791518