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MABN318 Anti-Transmembrane Prostatic Acid Phosphatase Antibody/TMPAP, clone 3G10.1

This Anti-Transmembrane Prostatic Acid Phosphatase Antibody/TMPAP, clone 3G10.1 is validated for use in western blotting & IHC (Paraffin) for the detection of Transmembrane Prostatic Acid Phosphatase/TMPAP.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: ACPP UniProt Number: P15309
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MABN318
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN318
      DescriptionAnti-Transmembrane Prostatic Acid Phosphatase Antibody/TMPAP, clone 3G10.1
      Alternate Names
      • Prostatic acid phosphatase
      • PAP
      • 5'-nucleotidase
      • 5'-NT
      • Ecto-5'-nucleotidase
      • Thiamine monophosphatase
      • Transmembrane Prostatic Acid Phosphatase
      • TMPase
      • ACPP
      • TMPAP
      Background InformationACPP, also known as Prostatic acid phosphatase, or PAP, 5'-nucleotidase (5'-NT), Ecto-5'-nuclotidase, or Thiamine monophosphatase (TMPase) , and encoded by the gene ACPP, is a non-specific tyrosine phosphatase that has both a secreted form and a cell membrane bound form. ACPP dephosphorylates a diverse number of substrates including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. ACPP also has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Additionally, the membrane bound form of ACPP has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons and acts as a pain suppressor. Finally ACPP can also act as a tumor suppressor of prostate cancer. ACPP isoform 1is secreted and isoform 2 is membrane bound. Isoform 2 appears to shuttle between the cell membrane, lysosomes, and intracellular vesicles. ACPP is expressed in many tissues and is used as a marker in various stages of prostatic cancer diagnostics. Also the enzyme was intensely studied in HIV infection because it can be proteolytically cleaved in seminal fluid to produce several peptides (SEVI peptides) one of which, PAPf39, happens to enhance HIV infection by binding HIV virions and helping to attach them to target cells--interestingly such SEVI peptides are degraded in vitro by EGCG, a compound found in green tea extract.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Transmembrane Prostatic Acid Phosphatase Antibody/TMPAP, clone 3G10.1 is validated for use in western blotting & IHC (Paraffin) for the detection of Transmembrane Prostatic Acid Phosphatase/TMPAP.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Transmembrane Prostatic Acid Phosphatase/TMPAP in human prostate tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Transmembrane Prostatic Acid Phosphatase/TMPAP.
      Clone3G10.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody is expected to cross react with isoform 2 based upon immunogen design.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ACPP
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~48 kDa observed. Two isoforms are known to exist at 45 kDa (isoform 1) and 48 kDa (isoform 2). Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human prostate tumor tissue lysate.

      Western Blotting Analysis: 4 µg/mL of this antibody detected this antibody detected Transmembrane Prostatic Acid Phosphatase/TMPAP in 200 µg of human prostate tumor tisssue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN318 04053252947056

      Documentation

      Anti-Transmembrane Prostatic Acid Phosphatase Antibody/TMPAP, clone 3G10.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Transmembrane Prostatic Acid Phosphatase Antibody/TMPAP, clone 3G10.1 Certificates of Analysis

      TitleLot Number
      Anti-Transmembrane Prostatic Acid Phosphatase/TMPAP, clone 3G10.1 - Q2370487 Q2370487
      Anti-Transmembrane Prostatic Acid Phosphatase/TMPAP, clone 3G10.1 - 3199164 3199164
      Anti-Transmembrane Prostatic Acid Phosphatase/TMPAP, clone 3G10.1 - 4005414 4005414
      Anti-Transmembrane Prostatic Acid Phosphatase/TMPAP, clone 3G10.1 -2702182 2702182