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ABS2269-100UG Anti-TRPM4

Anti-TRPM4, Cat. No. ABS2269, is a rabbit polyclonal antibody that detects TRPM4 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

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ABS2269-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2269-100UG
      DescriptionAnti-TRPM4
      Alternate Names
      • Transient receptor potential cation channel subfamily M member 4
      • Calcium-activated non-selective cation channel 1
      • Long transient receptor potential channel 4
      • TrpC-4
      • LTrpC4
      • MLS2s
      • Melastatin-like 2
      Background InformationTransient receptor potential cation channel subfamily M member 4 (UniProt: Q9ESQ5; also known as Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4, LTrpC-4, LTrpC4, MLS2s, Melastatin-like 2) is encoded by the Trpm4 (also known as Ltrpc4) gene (Gene ID: 171143) in rat. TRPM4 is a multi-pass membrane protein with four cytoplasmic domains; six transmembrane domains; and four extracellular domains. Two isoforms of TRPM4 have been described that are produced by alternative splicing. Isoform 1 is highly expressed in the testis tissue with a moderate expression in brain, spleen, and thymus. Isoform 2 is expressed in the brain and spleen. It serves as a calcium-activated non-selective cation channel that mediates membrane depolarization. Although it is a calcium activated channel, it is impermeable to calcium. It mediates the transport of monovalent cations that leads to membrane depolarization. It is reported to participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL-2 production. TRPM4 is also involved in myogenic constriction of cerebral arteries. It plays a role in control of insulin secretion in pancreatic β-cells. It may also be involved in pacemaking activity or could cause irregular electrical activity under conditions of Calcium overload. It is known to affect Th1 and Th2 cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. This polyclonal antibody is reported toblock the channel vua binding to a region close to the channel porefrom extracellular space. Bu blocking TRPM4, it can downregulate TRPM4 surface expression and prevent hypoxia-induced cell swelling. (Ref.: Wei, S., et al. (2020). Front Cell Dev Bio. 8; 562584; Chen, B;et al. (2019). Pflugers Arch. 471(11-12); 1455-1466; Guinamard, R., et al. (2006). Hypertension. 48(4); 587-594; Launay, P., et al. (2002). Cell. 109(3); 397-407).
      References
      Product Information
      FormatPurified
      Control
      • MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94000+ and ever-growing portfolio of antibodies to meet your research needs in Apoptosis and cancer
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      PresentationPurified rabbit polyclonal antibody in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-TRPM4, Cat. No. ABS2269, is a rabbit polyclonal antibody that detects TRPM4 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesTested applications

      Immunocytochemistry Analysis: A representative lot detected TRPM4 in Immunocytochemistry application (Low, S.W., et. al. (2021). Sci Rep. 11(1):10411).

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected TRPM4 in rat spinal cord tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-conjugated linear peptide corresponding to 28 amino acids between transmembrane segment 5 and 6 from the C-terminal half of rat TRPM4.
      EpitopeC-terminal half
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Transient receptor potential cation channel subfamily M member 4 (TRPM4). It targets an epitope within 24 amino acids from the C-terminal, extracellular domain.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Trpm4
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~135 kDa observed; 135.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells transfected with Mouse TRPM4.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TRPM4 in lysate from HEK293 cells transfected with Mouse TRPM4.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2269-100UG 04065269048251