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ST1099 Anti-TORC2 (454-607) Rabbit pAb

Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

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ST1099-50UL
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      Description
      OverviewRecognizes the ~79-85 kDa TORC2 protein in primary hepatocytes, HepG2, NIH3T3, Min6, and FAO cells.
      Catalogue NumberST1099
      Brand Family Calbiochem®
      SynonymsAnti-Transducer of regulated cAMP response element-binding protein (CREB) 2, Anti-TORC2
      Application Data
      Detection of rat TORC2 by immunoblotting. Sample: Whole cell lysate from primary rat hepatocytes. Primary antibody: Anti-TORC2 (454-607) Rabbit pAb (Cat. No. ST1099) (1:2000). Detection: chemiluminescence.
      References
      ReferencesScreaton, R.A., et al. 2004. Cell 119, 61.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlPrimary rat hepatocytes, HepG2, NIH3T3, Min6, and FAO cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:2000, see comments)
      Immunoprecipitation (1:100, see comments)
      Application CommentsThis antibody has been used to detect endogenous TORC2 by immunoblotting. For immunoprecipitation, 4 µl antibody in a total volume of 400 µl has been tested. May also detect a protein at ~60 kDa, which may be a degradation product of TORC2. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 454-607 of mouse TORC2 fused to GST
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
      Global Trade Item Number
      Catalogue Number GTIN
      ST1099-50UL 04055977224436