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MABE1994-100UL Anti-TFAM Antibody, clone 20G2C12

Anti-TFAM, clone 20G2C12, Cat. No. MABE1994, is a mouse monoclonal antibody that detects Transcription factor A, mitochondrial (TFAM) and is tested for use in ChIP-seq, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

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MABE1994-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1994-100UL
      DescriptionAnti-TFAM Antibody, clone 20G2C12
      Alternate Names
      • Transcription factor A mitochondrial
      • mtTFA
      • Mitochondrial transcription factor 1
      • MtTF1
      • Transcription factor 6
      • TCF-6
      • Transcription factor 6-like 2
      Background InformationTranscription factor A, mitochondrial (UniProt: Q00059; also known as mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM) is encoded by the TFAM (also known as TCF6, TCF6L2) gene (Gene ID: 7019) in human. TFAM is a DNA binding protein that plays multiple roles in regulating mitochondrial DNA function. As a sequence-specific transcription factor, it binds upstream of the light strand promoter and heavy strand promoter 1 to activate initiation of transcription. It is a component of the mitochondrial transcription initiation complex (composed of TFB2M, TFAM and POLRMT). In this complex, TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. TFAM contains two HMG boxes (aa 50-118 and 155-219). Phosphorylation by protein kinase A within the HMG box 1 is shown to impair its DNA binding and promotes its degradation by AAA+ Lon protease. Mutations in TFAM gene are known to cause hepatocerebral type of mitochondrial DNA depletion syndrome that is characterized by severe intrauterine growth restriction, neonatal-onset hypoglycemia and liver dysfunction. In this disease hepatic pathology includes cirrhosis, steatosis and cholestasis that leads to liver failure. (Ref.: Wang, YE., et al. (2013). PLoS ONE. 8(8); e74513).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-TFAM, clone 20G2C12, Cat. No. MABE1994, is a mouse monoclonal antibody that detects Transcription factor A, mitochondrial (TFAM) and is tested for use in ChIP-seq, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • ChIP-seq
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesChIP-seq Analysis: A representative lot detected TFAM in ChIP-seq applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated TFAM in Immunoprecipitation applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).

      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TFAM in HeLa cells.

      Western Blotting Analysis: A representative lot detected TFAM in Western Blotting applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).

      Immunocytochemistry Analysis: A representative lot detected TFAM in Immunocytochemistry applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length recombinant human Transcription factor A, mitochondrial (TFAM).
      EpitopeUnknown
      Clone20G2C12
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 20G2C12 is a mouse monoclonal antibody that detects human mitochondrial Transcription Factor A (TFAM).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TFAM
      • TCF6
      • TCF6L2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~25 kDa observed; 29.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysates.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected TFAM in HeLa cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1994-100UL 04065265872744