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ABC1444 Anti-Sorting Nexin-11 Antibody

Anti-Sorting Nexin-11 Antibody is an antibody against Sorting Nexin-11 for use in Western Blotting, Immunoprecipitation.

Anti-Sorting Nexin-11 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Vesicular Trafficking Gene Symbol: Snx11 UniProt Number: Q91WL6
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ABC1444
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1444
      DescriptionAnti-Sorting Nexin-11 Antibody
      Alternate Names
      • Sorting nexin-11
      • SNX11
      Background InformationSorting nexin-11 (UniProt Q91WL6) is encoded by the Snx11 gene (Gene ID 74479) in murine species. Sorting nexins (SNXs) are involved in diverse intracellular endosomal traffickings. Their phox homology (PX) domain binds certain phosphatidylinositols (PIs) and mediates their recruitment to PI-rich vesicles. Most SNXs contain additional recognizable domains and are referred to as SNX PX-BAR or SNX PX-other, while SNX3, SNX10, SNX11, SNX12, SNX22, and SNX24 contain only the PX domain and are referred to as SNX PX. SNX11 is found to contain a novel PXe domain (a.a. 16-132 of human and mouse SNX11) with two additional α-helices next to the conventional PX domain structure composed of three β-strands and three α-helices. F2RL1 (a.k.a. PAR2) is a protease-activated GPCR highly expressed throughout the central and the peripheral nervous system. F2RL1 is reported to translocate from the plasma membrane to nucleus upon stimulation via a microtubule-dependent shuttle machinery that requires sorting nexin 11.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Sorting Nexin-11 Antibody is an antibody against Sorting Nexin-11 for use in Western Blotting, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: 3.75 µg/mL from a representative lot detected sorting nexin-11 in 10 µg of HEK293T cell lysate.
      Western Blotting Analysis: A representative lot detected reducing amount of sorting nexin-11 in lysates from HEK293 cells that had been transfected with SNX11 shRNA (Joyal, J.S., et al. (2014). Nat. Med. 20(10):1165-1173).
      Western Blotting Analysis: A representative lot detected the presence of sorting nexin-11 in F2RL1-GFP fusion affinity pull-down only when using lysates of F2RL1-GFP-transfected HEK293 cells that had been stimulated with the F2RL1 agonist peptide (F2RL1-AP) SLIGKV, but not unstimulated F2RL1-GFP transfectants (Joyal, J.S., et al. (2014). Nat. Med. 20(10):1165-1173).
      Immunoprecipitation Analysis: A representative lot co-immunoprecipitated importin-β1 (Impβ1) and F2RL1 with sorting nexin-11 from lysates of HEK293 cells that had been stimulated with the F2RL1 agonist peptide (F2RL1-AP) SLIGKV (Joyal, J.S., et al. (2014). Nat. Med. 20(10):1165-1173).
      Biological Information
      ImmunogenLinear peptide corresponding to an internal sequence from the C-terminal half of mouse sorting nexin-11.
      EpitopeInternal (C-terminal half).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody reacts with both human and murine sorting nexin-11. The immunogen sequence is also present in human spliced isoform 2 and rat sorting nexin-11.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Snx11
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~35 kDa observed. 30.43 kDa (human & mouse isoform 1) and 29.42 kDa (human isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 membrane extract.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected sorting nexin-11 in 10 µg of MCF-7 membrane extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1444 04055977335187