Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Species
Panel Type
Selected Kit
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
96-Well Plate
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
This item has been added to favorites.
The Product Has Been Added To Your Cart
You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
ABT257
Sigma-AldrichAnti-Pro-Collagen Type I, A1/COL1A1
Anti-Pro-Collagen Type I Antibody is an antibody against Pro-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.
More>>Anti-Pro-Collagen Type I Antibody is an antibody against Pro-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry. Less<<
Anti-Pro-Collagen Type I, A1/COL1A1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Collagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human. Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain. Alpha-1 type I collagen is initially produced as an 1464-amino acid prepro-form with a signal peptide sequence (a.a. 1-22) and two propeptide sequences (a.a. 23-161 and a.a. 1219 –1464), the removal of which yields the mature alpha-1(I) chain. The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a. 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a. 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a. 1193-1218; numbering based on the prepro-form). Collagen can be extracted from tissue via either enzymatic or non-enzymatic means. Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin. On the other hand, collagen preparations obtained with non-enzymatic means (e.g. by acid extraction) have the intact telopeptides at both ends.
References
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Anti-Pro-Collagen Type I Antibody is an antibody against Pro-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.
Key Applications
Western Blotting
Flow Cytometry
Immunohistochemistry
Application Notes
Western Blotting Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung and skin tissue lysate. Flow Cytometry Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung tissue. Immunohistochemistry Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung tissue.
Biological Information
Immunogen
Linear peptide corresponding to sequence derived from human Pro-Collagen Type I, A1/COL1A1 C-terminal propeptide region.
Epitope
C-terminal propeptide region
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
Cat. No. ABT257 Anti-Pro-Collagen Type I, A1/COL1A1 was raised against a sequence derived from the C-terminal propeptide (not the N-terminal propeptide) region. This polyclonal antibody will detect prepro- and pro-, but not mature collagen alpha-1(I) chain.
Species Reactivity
Human
Mouse
Species Reactivity Note
Human, Mouse. Predicted to react with a broad range of species based on high sequence homology, including mammalian, avian, fish, and amphibian species.
Evaluated by Western Blotting in mouse skin tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Pro-Collagen Type I, A1/COL1A1 in 10 µg of mouse skin tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
