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MABF2250-100UL Anti-NKp44 (CD336) Antibody, clone 3.43.13

MABF2250-100UL
100 µL  
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      Overview

      Description
      Catalogue NumberMABF2250-100UL
      DescriptionAnti-NKp44 (CD336) Antibody, clone 3.43.13
      Alternate Names
      • Natural cytotoxicity triggering receptor 2
      • Lymphocyte antigen 95 homolog
      • NK cell-activating receptor
      • Natural killer cell p44-related protein
      • NK-p44
      • NKp44
      • CD336
      Background InformationNatural cytotoxicity triggering receptor 2 (UniProt: O95944; also known as Lymphocyte antigen 95 homolog, NK cell-activating receptor, Natural killer cell p44-related protein, NK-p44, NKp44, CD336) is encoded by the NCR2 (also known as LY95) gene (Gene ID: 9436) in human. NKp44 is an activating receptor expressed on human NK cells and its expression is induced upon activation by interleukin-2. Its expression is not observed on the surface of resting NK cells. It can induce cytokine production and cytolytic activity in human NK cells. NKp44 consists of a single extracellular V-type Ig domain, a transmembrane region, and a short cytoplasmic. Through its transmembrane region, it associates with the adaptor molecule DNAX activation protein 12 (DAP12) that contains an immunoreceptor tyrosine-based activation motif (ITAM) and DAP12 is required for NKp44 cell-surface expression and downstream signaling events. NKp44 is reported to mediate cytotoxicity against various tumor cells, including neuroblastoma and choriocarcinoma. In human NK-like cell line NKp44 exhibits strong redirected cytotoxicity and modest IFN-gamma production. (Ref.: Fuchs, A., et al. (2005). Blood. 115(6); 2076-2082; Kikuchi-Maki. A., et al. (2003). J. Immunol. 171(7); 3415-3425).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without any preservatives.
      Applications
      ApplicationAnti-NKp44 (CD336), clone 3.43.13, Cat. No. MABF2250, is a mouse monoclonal antibody that detets Natural cytotoxicity triggering receptor 2 (NKp44) and has been tested for use in Flow Cytometry, Function Analysis, and Immunocytochemistry.
      Key Applications
      • Flow Cytometry
      • Affects Function
      • Immunocytochemistry
      Application NotesAffects Function Analysis: A representative lot activated both NK92-44wt and NK92-44mut cell lines (Brusilovsky, M., et. al. (2015). Eur J Immunol. 45(4):1180-91).


      Immunocytochemistry Analysis: A representative lot detected NKp44 (CD336) in Immunocytochemistry applications (Brusilovsky, M., et. al. (2015). Eur J Immunol. 45(4):1180-91).

      Flow Cytometry Analysis: A representative lot detected NKp44 (CD336) in Flow Cytometry applications Barrow, A.D., et. al. (2018). Cell. 172(3):534-548; Kikuchi-Maki, A., et. al. (2003). J Immunol. 171(7):3415-25; Fuchs, A., et. al. (2005). Blood. 106(6):2076-82; Brando, C., et. al. (2005). J Leukoc Biol. 78(2):359-71; Eisenberg, V., et. al. (2017). Front Immunol. 8:1212).
      Biological Information
      ImmunogenNKp44-human crystallizable fragment (Fc) fusion protein.
      Clone3.43.13
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3.43.13 is a mouse monoclonal antibody that detects human Natural cytotoxicity triggering receptor 2 (NKp44) protein.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NCR2
      • LY95
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight30.68 kDa calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in rhIL-2 stimulated human peripheral blood mononuclear cells (PBMCs) for 7 days (100 U per mL).

      Flow Cytometry Analysis: 1 µg of this antibody detected NKp44 (CD336) in rhIL-2 stimulated one million human peripheral blood mononuclear cells (PBMCs) for 7 days (100 U per mL).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2250-100UL 04054839961694