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06-886-I-25UG Anti-NFκB p50 (rabbit polyclonal)

Anti-NFκB p50 Antibody is an antibody against NFκB p50 for use in Western Blotting, Immunoprecipitation.

Anti-NFκB p50 (rabbit polyclonal) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: NFKB1 UniProt Number: P19838
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06-886-I-25UG
25 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-886-I-25UG
      DescriptionAnti-NFκB p50 (rabbit polyclonal)
      Alternate Names
      • Nuclear factor NF-kappa-B p105 subunit
      • NFκB p50
      • DNA-binding factor KBF1
      • EBP-1
      • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
      Background InformationNuclear factor NF-kappa-B p105 subunit (UniProt P19838; also known as DNA-binding factor KBF1, EBP-1, NF-kappa-B, NF-kappaB, NF-kB1, NFkappaB, NFKB-p105, NFKB-p50, Nuclear factor NF-kappa-B p50 subunit, Nuclear factor kappa-B DNA binding subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1) is encoded by the NFKB1 gene (Gene ID 4790) in human. NF-κB is a protein complex that plays a key role in regulating the immune response to infection/inflammation. NF-κB1 is synthesized as a 968 a.a protein (p105), which undergoes posttranslational processing to generate the p50 subunit (a.a. 1-433). The p50 NF-κB participates in target gene transactivation by forming heterodimers with RelA, RelB, or c-Rel. In addition, p50 homodimer can bind the nuclear protein Bcl-3 and form a functional transcriptional activator complex. In unstimulated cells, the NF-κB dimers are sequestered in the cytoplasm by the family of IκB (Inhibitor of κB) proteins (IκBα, IκBβ, IκBε, and Bcl-3). Upon stimulation, IκB becomes phosphorylated by the IκB kinase (IKK) complex, leading to IκB ubiquitination and proteasomal degradation. With the degradation of IκB, the NF-κB complex is then freed to enter the nucleus to activate the transcription of target genes.
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NFκB p50 Antibody is an antibody against NFκB p50 for use in Western Blotting, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 µg of antibody from a representative lot immunoprecipitated NFkB p50 in 500 µg of A431 cell lysate.
      Biological Information
      ImmunogenKLH conjugated synthetic peptide (MAEDDPYLGRPE-C) corresponding to amino acids 1-12 of NFκB p50, containing a C-terminal cysteine.
      EpitopeAmino acid 1-12
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityDectects 105 kDa recursor and 50 kDa processed forms. Immunogen sequence is a.a. 1-12, this polyclonal antibody can therefore detect isoforms 1 and 2, but not 3. Note that isoform 3 is missing amino acids 1-180.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NFKB1
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~50/105 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected NFkB p50 in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Global Trade Item Number
      Catalogue Number GTIN
      06-886-I-25UG 04054839337314