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MABS2161-100UG Anti-NARC-1 (PCSK9) Antibody, clone 15A6

MABS2161-100UG
100 μg  
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      Overview

      Description
      Catalogue NumberMABS2161-100UG
      DescriptionAnti-NARC-1 (PCSK9) Antibody, clone 15A6
      Alternate Names
      • Proprotein convertase subtilisin/kexin type 9
      • Neural apoptosis-regulated convertase 1
      • Proprotein convertase 9
      • PC9
      • Subtilisin/kexin-like protease PC9.
      Background InformationProprotein convertase subtilisin/kexin type 9 (UniProt: Q8NBP7; also known as Neural apoptosis-regulated convertase 1, NARC-1, Proprotein convertase 9, PC9, Subtilisin/kexin-like protease PC9) is encoded by the PCSK9 (also known as NARC1, PSEC0052) gene (Gene ID: 255738) in human. NARC-1 (PCSK9) is a monomeric protein that can also self-associate to form dimers and higher multimers. It plays an important role in the regulation of cholesterol homeostasis by binding to hepatic low-density lipoprotein receptors (LDL-R) and promote their lysosomal degradation. Higher multimers display increased LDL-R degrading activity. PCSK9 is synthesized with a signal peptide (aa 1-30) and a propeptide (aa 31-152), which are cleaved off in the mature active form. Its proteolytic activity is autoinhibited by the non-covalent binding of the propeptide to the catalytic domain. Its C-terminal domain (aa 450-692) is essential for the LDL-R degradation activities. The cleaved propeptide remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein. PCSK9 can also undergo phosphorylation, which protects the propeptide against proteolysis. Mutations in PCSK9 gene leading to gain-of-function are known to cause hypercholesterolemia and those with loss-of-function can diminish LDL levels and reduce cardiovascular disease risk. (Ref.: Bergeron, N, et al. (2015). Circulation. 132(17); 1648-1666).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-NARC-1 (PCSK9), clone 15A6, Cat. No. MABS2161, is a mouse monoclonal anitbody that detects Proprotein convertase subtilisin/kexin type 9 and has been tested for use in ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected NARC-1 (PCSK9) in Western Blotting applications (Zhang, D.W., et. al. (2008). Proc Natl Acad Sci USA. 105(35):13045-50; Laggace, T.A., et. al. (2006). J Clin Invest. 116(11):2995-3005; Zhang, D.W., et. al. (2007). J Biol Chem. 282(25):18602-12).

      Immunocytochemistry Analysis: A representative lot detected NARC-1 (PCSK9) in Immunocytochemistry applications (Zhang, D.W., et. al. (2007). J Biol Chem. 282(25):18602-12).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated NARC-1 (PCSK9) in Immunoprecipitation applications (Zhang, D.W., et. al. (2007). J Biol Chem. 282(25):18602-12).

      ELISA Analysis: A representative lot detected NARC-1 (PCSK9) in ELISA applications (Laggace, T.A., et. al. (2006). J Clin Invest. 116(11):2995-3005; Lakoski, S.G., et. al. (2009). J Clin Endocrinol Metab. 94(7):2537-43).
      Biological Information
      ImmunogenFull-length purified human Proprotein convertase 9 (PCSK9).
      Clone15A6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 15A6 is a mouse monoclonal antibody that specifically detects Proprotein convertase 9 (NARC-1) and its cleaved form in human cells.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PCSK9
      • NARC1
      • PSEC0052
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~80 and 60 kDa observed (proprotein and cleaved form); 74.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human lung tissue lysates.

      Western Blotting Analysis: 4 µg/mL of this antibody detected NARC-1 (PCSK9) in human lung tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2161-100UG 04054839997419