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444905 Anti-Mast Cell Tryptase Mouse mAb (AA1)

This Anti-Mast Cell Tryptase Mouse mAb (AA1) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Mast Cell Tryptase.

Anti-Mast Cell Tryptase Mouse mAb (AA1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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444905
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ca, Fe, H, MkMMonoclonal Antibody

Pricing & Availability

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444905-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the 32-38 kDa α- and β-isoforms of tryptase in mast cells.
      Catalogue Number444905
      Brand Family Calbiochem®
      Application Data
      Detection of human mast cell tryptase by immunohistochemistry. Sample: Human tonsil. Priamry antibody: Anti-Mast Cell Tryptase Mouse mAb (AA1) (Cat. No. 444905) (1:2000). Detection: fluorescence.
      References
      ReferencesSommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
      Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341.
      Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111.
      Walls, A.F., et al. 1990. J. Pathol. 162, 119.
      Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Positive controlCat, dog, human, or monkey mast cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen Sections (0.06-0.12 µg/ml)
      Immunoblotting (1 µg/ml)
      Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
      Application CommentsStaining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogennative, human mast cell, tryptase
      ImmunogenHuman
      CloneAA1
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Canine
      • Feline
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      444905-100UG 04055977186208

      Documentation

      Anti-Mast Cell Tryptase Mouse mAb (AA1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Mast Cell Tryptase Mouse mAb (AA1) Certificates of Analysis

      TitleLot Number
      444905

      References

      Reference overview
      Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
      Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341.
      Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111.
      Walls, A.F., et al. 1990. J. Pathol. 162, 119.
      Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-October-2008 JSW
      ApplicationFrozen Sections (0.06-0.12 µg/ml)
      Immunoblotting (1 µg/ml)
      Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
      Application Data
      Detection of human mast cell tryptase by immunohistochemistry. Sample: Human tonsil. Priamry antibody: Anti-Mast Cell Tryptase Mouse mAb (AA1) (Cat. No. 444905) (1:2000). Detection: fluorescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~32-38 kDa α- and β-isoforms of tryptase in mast cells.
      BackgroundMast cells are tissue bound cells that normally are distributed throughout connective tissues. They are involved in inflammatory processes as important effector and regulatory cells. Mast cells are known to accumulate at sites of inflammation, such as asthma, allergic rhinitis, and rheumatoid arthritis. Mast cells contain preformed chemical mediators including histamine, chymase, carboxypeptidase and proteolytic tryptase. Mast cells of the skin and lung contain large amounts of tryptase, suggesting this enzyme has a major role in mast cell-mediated events. Human mast cell tryptase has the potential to be used as marker for mast cell activation and as a mediator of inflammation.
      HostMouse
      Immunogen speciesHuman
      Immunogennative, human mast cell, tryptase
      CloneAA1
      IsotypeIgG₁
      Speciescanine, feline, human, monkey
      Positive controlCat, dog, human, or monkey mast cells
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsStaining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
      Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341.
      Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111.
      Walls, A.F., et al. 1990. J. Pathol. 162, 119.
      Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.