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MABC1636-25UL Anti-MLKL Antibody, clone 7G2

Anti-MLKL, clone 7G2, Cat. No. MABC1636, is a rat monoclonal antibody that detects MLKL and is tested for use in Immunofluorescence and Western Blotting.

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MABC1636-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1636-25UL
      DescriptionAnti-MLKL Antibody, clone 7G2
      Alternate Names
      • Mixed lineage kinase domain-like protein
      Background InformationMixed lineage kinase domain-like protein (UniProt: Q8NB16; also known as hMLKL) is encoded by the MLKL gene (Gene ID: 197259) in human. MLKL is a pseudokinase that is a terminal protein in the pro-inflammatory necroptotic cell death program. It plays a critical role in TNFa-induced necroptosis. Although it contains a protein kinase domain (aa 194-469), it lacks several residues that are essential for protein kinase activity. It is activated following phosphorylation at Thr357/Ser358 by RIPK3, which leads to its homotrimerization and localization to the plasma membrane where it binds to highly phosphorylated inositol (InsP6) and cardiolipin and disrupts membrane integrity to cause necrotic cell death. Under basal conditions, MLKL is shown to reside in small puncta that are distributed evenly throughout the cytoplasm, but it is detected in plasma membrane shortly after its phosphorylation. It contains two coiled coil regions (aa 55-84 and 139-180). The second coiled coil region is responsible for its oligomerization. MLKL is also reported to be involved in endosomal trafficking, independently of induction of necroptosis, whereby it enhances degradation of receptors and ligands. Two isoforms of MLKL have been described that are produced by alternative splicing. (Ref.: Samson, AL., et al. (2020). Nat. Commun. 11(1); 3151; Yoon, S., et al. (2017). Immunity. 47(1); 51-65; Wang, H., et al (2014). Mol. Cell 54(1); 133-146).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-MLKL, clone 7G2, Cat. No. MABC1636, is a rat monoclonal antibody that detects MLKL and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected MLKL in Western Blotting applications (Samson, A.L., et al. (2020). Nat Commun. 11(1):3151).

      Immunofluorescence Analysis: A representative lot detected MLKL in Immunofluorescence applications (Samson, A.L., et al. (2020). Nat Commun. 11(1):3151).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 282 amino acids from human Mixed lineage kinase domain-like protein (MLKL) expressed in baculovirus expression system.
      EpitopeC-terminal
      Clone7G2
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 7G2 is a rat monoclonal antibody that detects Human Mixed lineage kinase domain-like protein (MLKL). It targets an epitope within the C-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MLKL
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~50 kDa observed; 54.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from HT-29 cells.

      Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected MLKL in lysates from wild-type HT-29 cells, but not in MLKL knockout cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1636-25UL 04065268041987