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MAB19562-AF647 Anti-Laminin-5 Antibody (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate

Anti-Laminin-5 Antibody (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate is an antibody against Laminin-5 for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: LAMC2, LAMB2T, LAMNB2 UniProt Number: Q13753-1
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MAB19562-AF647
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICCMAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue NumberMAB19562-AF647
      DescriptionAnti-Laminin-5 Antibody (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate
      Alternate Names
      • Laminin subunit gamma-2
      • Cell-scattering factor 140 kDa subunit
      • CSF 140 kDa subunit
      • Epiligrin subunit gamma
      • Kalinin subunit gamma
      • Kalinin/nicein/epiligrin 100 kDa subunit
      • Ladsin 140 kDa subunit
      • Laminin B2t chain
      • Laminin-5 subunit gamma
      • Large adhesive scatter factor 140 kDa subunit
      • Nicein subunit gamma
      • Laminin-5 (γ2 chain)
      Background InformationLaminin subunit gamma-2 (UniProt Q13753; also known as CSF 140 kDa subunit, Cell-scattering factor 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Ladsin 140 kDa subunit, Laminin B2t chain, Lamin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as B2T, LAMB2T, LAMNB2) gene (Gene ID 3918) in human. Laminins are basement membrane, extracellular matrix glycoproteins composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes. Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease. LN5 may stabilize the epidermal/dermal junction of skin through interaction with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. Comparing to fibronectin, collagen, and vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent. Laminin-5 attracted attention when it was detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, T., et al. (2002). Am. J. Pathol. 160(3):112911-41; Lohi, J. (2001). Int. J. Cancer. 94(6):763-767).
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified mouse antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Laminin-5 Antibody (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate is an antibody against Laminin-5 for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Laminin-5 in NIH/3T3 cells.
      The unconjugated antibody (Cat. No. MAB19562) is shown to be suitable also for Western blotting, ELISA, and immunohistochemistry applications.
      Biological Information
      ImmunogenAmino acid residues 382-608 of human laminin-5 gamma2 chain (III domain) expressed as a GST fusion protein.
      EpitopeEGF-like 4 (second part)
      CloneD4B5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThis antibody recognizes the 150 (precursor form) and 105 kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LAMC2
      • LAMB2T
      • LAMNB2
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~150 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Laminin-5 in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB19562-AF647 04055977301144

      Documentation

      Anti-Laminin-5 Antibody (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Laminin-5 Antibody (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-Laminin-5 (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate - 3218752 3218752
      Anti-Laminin-5 (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate - 3452492 3452492
      Anti-Laminin-5 (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate - 3911625 3911625
      Anti-Laminin-5 (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate - 4124923 4124923
      Anti-Laminin-5 (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate -Q2632619 Q2632619