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MABS489 Anti-LRP-2/Megalin Antibody, clone 10D5.1

Anti-LRP-2/Megalin Antibody, clone 10D5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Lipid Signaling Gene Symbol: LRP2, DBS UniProt Number: P98164
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MABS489
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS489
      DescriptionAnti-LRP-2/Megalin Antibody, clone 10D5.1
      Alternate Names
      • Low-density lipoprotein receptor-related protein 2
      • Calcium sensor protein
      • Glycoprotein 330
      • gp330
      • LRP-2
      • Megalin
      Background InformationLow-density lipoprotein receptor-related protein 2 (UniProt P98164; also known as Calcium sensor protein, Glycoprotein 330, gp330, Heymann nephritis antigen homolog, LRP-2, Megalin) is encoded by the LRP2 (also known as DBS) gene (Gene ID 4036) in human. Megalin/LRP-2 is a 600 kDa type I membrane receptor initially produced with a signal peptide sequence (a.a. 1-25). The mature receptor has a large extracellular region (a.a. 26-4423), a transmembrane segment (a.a. 4424-4446), and cytoplasmic tail (a.a. 4447-4655). Megalin belongs to the LDLR family of membrane glycoproteins whose members also inculde the low-density lipoprotein receptor (LDLR), the very-low-density lipoprotein (VLDL) receptor, the LDLR-related protein (LRP), and the apolipoprotein E receptor 2 (ApoER2). The overall extracellular structural features are similar among all LDLR family members. Megalin contains four clusters of cysteine-rich Complement-type/LDLR Class A repeats that mediate ligand-binding. These are separated by 17 EGF-like repeats and eight cysteine-poor spacer regions that contain YWTD motifs involved in the pH-dependent ligands dissociation in endosomal compartments. Megalin is a multiligand, endocytic receptor expressed primarily in the apical membranes of polarized epithelial cells. In addtion to functioning alone, megalin is also reported to interact with another structurally distinct endocytic receptor cubilin in several tissues where they are co-expressed. The internalization of several cubilin ligands is reported to be strongly facilitated in the presence of megalin.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected LRP-2/Megalin in 10 µg of human kidney tissue lysate.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the LDL-receptor class A repeats 19-21 of human LRP-2/Megalin.
      EpitopeLDL-receptor class A repeats 19-21.
      Clone10D5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LRP2
      • DBS
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~250 to 600 kDa observed. A broad/smeared banding pattern is consistent with the detection of the target protein with varying degrees of glycosylation (520 kDa/non-glycosylated to 660 kDa/fully glycosylated), as well as smaller size degradation products.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human kidney tissue.

      Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected LRP-2/Megalin in human kidney tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS489 04055977311754

      Documentation

      Anti-LRP-2/Megalin Antibody, clone 10D5.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-LRP-2/Megalin Antibody, clone 10D5.1 Certificates of Analysis

      TitleLot Number
      Anti-LRP-2/Megalin, clone 10D5.1 - 3276064 3276064
      Anti-LRP-2/Megalin, clone 10D5.1 - 3425385 3425385
      Anti-LRP-2/Megalin, clone 10D5.1 - 3880374 3880374
      Anti-LRP-2/Megalin, clone 10D5.1 - 3978175 3978175
      Anti-LRP-2/Megalin, clone 10D5.1 - 4074796 4074796
      Anti-LRP-2/Megalin, clone 10D5.1 - 4206972 4206972
      Anti-LRP-2/Megalin, clone 10D5.1 -Q2636947 Q2636947
      Anti-LRP-2/Megalin, clone 10D5.1 Monoclonal Antibody 2938796
      Anti-LRP-2/Megalin, clone 10D5.1 Monoclonal Antibody 3013240