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MABT1456-25UL Anti-LAMC2 Antibody, clone F2H4

This mouse monoclonal Anti-LAMC2, clone F2H4, Cat. No. MABT1456, is a mouse monoclonal antibody that detects Laminin subunit gamma-2 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

MABT1456-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1456-25UL
      DescriptionAnti-LAMC2 Antibody, clone F2H4
      Alternate Names
      • Laminin subunit gamma-2
      • Cell-scattering factor 140 kDa subunit
      • CSF 140 kDa subunit
      • Epiligrin subunit gamma
      • Kalinin subunit gamma
      • Kalinin/nicein/epiligrin 100 kDa subunit
      • Ladsin 140 kDa subunit
      • Laminin B2t chain
      • Laminin-5 subunit gamma
      • Large adhesive scatter factor 140 kDa subunit
      • Nicein subunit gamma
      Background InformationLaminin subunit gamma-2 (UniProt: Q13753; also known as Cell-scattering factor 140 kDa subunit, CSF 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as LAMB2T, LAMNB2) gene (Gene ID: 3918) in human. Laminin is a major basement membrane glycoprotein consisting of a, b, and g chains that are bound to each other by disulfide bonds. Over 15 different isoforms have been reported in the laminin family that are expressed in a tissue-specific manner and play different role in each tissue. g 2, a subunit of Laminin-5 (also known as Laminin-332) is reported to be frequently over-expressed as a monomer or as the b3- g 2 heterodimer in invasive cancers and is regarded as an invasion marker. It has 8 EGF-like domains. The cell adhesion and motility activities of Laminin-5 are reported to be regulated by the proteolytic cleavage of the short arm of Laminin g 2 chain by BMP-1 or MT1-MMP. This cleavage releases a 45 kDa N-terminal proteolytic fragment (g 2pf). The short arm and g 2pf can be further cleaved by serine proteases and by matrix metalloproteinases resulting in the release of small N-terminal fragments. g 2pf‐like fragments have been reported to be present in conditioned media of cultured cancer cells and in sera from human cancer subjects. Clone F2H4 targets an epitope within NE1 of domain V of Laminin g 2 and shows reactivity with Laminin g 2, g 2pf and N-terminal fragments. (Ref.: Sato, H., et al. (2014). Cancer Sci. 105(2); 168-175; Miyazaki, K., et al. (2016). Cancer Sci. 107(12); 1909-1918).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-LAMC2, clone F2H4, Cat. No. MABT1456, is a mouse monoclonal antibody that detects Laminin subunit gamma-2 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • ELISA
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected LAMC2 in Purified Lmg2pf and purified Lmg2 full length protein (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

      Immunohistochemistry Applications: A representative lot detected LAMC2 in Immunohistochemistry applications in Squamous cell carcinoma of the uterus and lung carcinoma tissues (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

      ELISA Analysis: 5 µg/mL from a representative lot detected LAMC2 in Lm-gamma2pf and Lm-gamma2 in Lm332 condition medium from Lm332-expressing HEK293 cells (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged full-length human Laminin subunit gamma-2 (LAMC2).
      EpitopeUnknown
      CloneF2H4
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone F2H4 is a mouse monoclonal antibody that specifically detects human Laminin subunit gamma-2 (LAMC2). It targets an epitope within the NE1 repeat of domain V.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LAMC2
      • LAMB2T
      • LAMNB2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight130.98 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected LAMC2 in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1456-25UL 04065265398060