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05-1154 Anti-JAK1 Antibody, clone 73

Anti-JAK1 Antibody, clone 73 detects level of JAK1 & has been published & validated for use in WB, IP & IF.

Anti-JAK1 Antibody, clone 73 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Immunological Signaling Gene Symbol: JAK1, JAK1B, JAK-1, JAK1A, EC 2.7.10.2 UniProt Number: P23458
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05-1154
100 µg  
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      Overview

      Replacement Information
      Replacement Information05-1154 is a recommended replacement for MAB3700

      Special Offers

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Ca, Xn, ChWB, IP, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1154
      ReplacesMAB3700
      DescriptionAnti-JAK1 Antibody, clone 73
      Alternate Names
      • Janus kinase 1
      • Janus kinase 1 (a protein tyrosine kinase) 1
      • Tyrosine-protein kinase JAK1
      Background InformationJAKs are non-receptor tyrosine kinases which couple to cytokine receptors, and are activated by cytokines. They regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. JAK kinases (JAK1, JAK2, JAK3, Tyk2) have a kinase domain and a second, pseudokinase domain which is not enzymatically active. The principal effectors of JAKs are the STATs (Signal Transducers and Activators of Transcription), which are direct activators of transcription, but JAKs also signal to the Ras-MAP Kinase pathway, and through Tec, to the PI3 Kinase pathway. Immunological stimuli, such as interferons and cytokines, induce recruitment of STAT transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal STAT transcription factors, which subsequently dimerize and translocate to the nucleus where they bind DNA. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 (Tyr1007/1008) are sites of trans- or autophosphorylation in vivo and in vitro kinase reactions. JAK2 is required for signaling through a different subset of cytokine receptors, while JAK1 is essential for signaling from the IL-2, IL-6 and interferon receptors. JAK3 is required for signaling through cytokine receptors which share a common gamma-chain.
      References
      Product Information
      FormatPurified
      Control
      • Huvec cell lysate, whole Jurkat cell lysate
      PresentationPurified mouse monoclonal IgG2b aqueous buffered solution containing 50% glycerol, BSA, and < 0.09% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-JAK1 Antibody, clone 73 detects level of JAK1 & has been published & validated for use in WB, IP & IF.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunofluorescence
      Application NotesImmunoprecipitation: A previous lot of this antibody was used in immunoprecipitation under native and denatured conditions.

      Immunofluorescence: A previous lot of this antibody was used in IF.
      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenGenerated from human JAK1, amino acids 551-776.
      Clone73
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes JAK1 (Janus Kinase 1). The members of the JAK family of cytoplasmic protein tyrosine kinases physically associate with ligand-bound receptors. It is this association that results in tyrosine phosphorylation and activation. JAK1 is approximately 130 kDa and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members.
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Canine
      • Xenopus
      • Chicken
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryJanus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.
      Gene Symbol
      • JAK1
      • JAK1B
      • JAK-1
      • JAK1A
      • EC 2.7.10.2
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P23458 # Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
      SIZE: 1142 amino acids; 131957 Da
      SUBUNIT: Interacts with IL31RA and SHB.
      SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane protein. Note=Wholly intracellular, possibly membrane associated.
      DOMAIN: SwissProt: P23458 Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain.
      Molecular Weight130 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Huvec lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected JAK1 on 10 μg of Huvec lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable frozen at -20°C in undiluted aliquots for up to 1 year from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-1154 04053252735509

      Documentation

      Anti-JAK1 Antibody, clone 73 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-JAK1 Antibody, clone 73 Certificates of Analysis

      TitleLot Number
      Anti-JAK1, clone 73 3035205
      Anti-JAK1, clone 73 - 2426532 2426532
      Anti-JAK1, clone 73 - 2204996 2204996
      Anti-JAK1, clone 73 - 2283924 2283924
      Anti-JAK1, clone 73 - 3552763 3552763
      Anti-JAK1, clone 73 - 3611144 3611144
      Anti-JAK1, clone 73 - 3720639 3720639
      Anti-JAK1, clone 73 - 3918082 3918082
      Anti-JAK1, clone 73 - 4284761 4284761
      Anti-JAK1, clone 73 - 4338974 4338974
      Show more

      Brochure

      Title
      Pathways and Biomarkers of JAK/STAT Signaling

      Technical Info

      Title
      JAK/STAT Signaling Research Focus

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies