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MABN2436 Anti-Guanylate Cyclase 2E Antibody, clone 8A5

Anti-Guanylate Cyclase-2E, clone 8A5, Cat. No. MABN2436, is a highly specific mouse monoclonal antibody that targets retinal Guanylyl cyclase (RETGC-1; GC-2E) and has been used in Immunofluorescence and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: GUCY2D UniProt Number: P52785
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MABN2436
200 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, MIF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2436
      DescriptionAnti-Guanylate Cyclase 2E Antibody, clone 8A5
      Alternate Names
      • RETGC-1
      • Guanylate cyclase-E
      • Guanylate cyclase-2E
      • GC-E
      Background InformationGuanylyl cyclase GC-E (UniProt: P52785; also known as EC: 4.6.1.2, Guanylate cyclase 2E, GC-2E) is encoded by the Gucy2e (also known as Guc2e) gene (Gene ID: 14919) in murine species. GC-2E is a single-pass type I membrane protein that plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the re-synthesis of cGMP required for recovery of the dark state after phototransduction. Following photoexcitation, cGMP in photoreceptors is first hydrolyzed by a light-activated phosphodiesterase (PDE6) and then replenished by a retinal membrane guanylyl cyclase. It is synthesized with a 50 amino acid signal peptide that is cleaved to generate the mature enzyme. It consists of an extracellular domain (aa 51-469), a helical domain (aa 470-490) and a cytoplasmic domain (aa 491-1108).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Guanylate Cyclase-2E, clone 8A5, Cat. No. MABN2436, is a highly specific mouse monoclonal antibody that targets retinal Guanylyl cyclase (RETGC-1; GC-2E) and has been used in Immunofluorescence and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected Guanylate Cyclase-1 in mouse retina tissues (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

      Immunofluorescence Analysis: A representative lot detected Guanylate Cyclase-1 in Immunofluorescence applications (Molday, L.L., et. al. (2013). Hum Mol Genet. 22(19):3894-905).

      Western Blotting Analysis: 10 ug/mL of a representative lot detected Guanylate Cyclase-1 in mouse retina tissue lysates (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

      Western Blotting Analysis: A representative lot detected Guanylate Cyclase-1 in Western Blotting applications (Peshenko, I.V., et. al. (2011). Biochemistry. 50(44):9511-9; Molday, L.L., et. al. (2013). Hum Mol Genet. 22(19):3894-905)
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 48 amino acids from the C-terminal end of human Guanylate cyclase-1.
      EpitopeC-terminus
      Clone8A5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 8A5 detects Guanylate cyclase-1 in Bovine, Human, and murine species. It targets an epitope within 48 amino acids from the C-terminal end.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Bovine
      • Mouse
      Species Reactivity NoteHuman, Bovine, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GUCY2D
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~120 kDa observed; 120.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2436 04054839339646