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MAB3194 Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH

Detect the Glutathione: N-ethylmaleimide adduct protein using this Anti-Glutathione: N-ethylmaleimide adduct, clone 8.1GSH validated for use in ELISA, Flow, ICC, IHC & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Oxidative Stress
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MAB3194
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MaELISA, FC, ICC, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3194
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationLiquid in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect the Glutathione: N-ethylmaleimide adduct protein using this Anti-Glutathione: N-ethylmaleimide adduct, clone 8.1GSH validated for use in ELISA, Flow, ICC, IHC & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry: 1:100 (paraformaldehyde fixation)

      Immunocytochemistry: 1:100 - 1:500 for assessment of intracellular GSH distribution.

      ELISA: 1:100,000 - 1:200,000 to quantify GSH at >2 mM in biological specimens.

      Flow cytometry: 1:100 - 1:1,000

      Cells were fixed with cold 1% PFA for 5 min and vortexed into methanol (-20°C) containing 5mM NEM (10 min on dry ice) or 10 μM NEM (N-ethyl maleimide) for 30 min at RT.

      Optimal working dilutions must be determined by end user.

      Positive Control: Peripheral blood mononuclear cells (PBMC) treated with NEM
      Biological Information
      ImmunogenGS-NEM conjugated to KLH
      Clone8.1GSH
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReactive with thiol-modified glutatione. Does not react with oxidized glutathione (GSSG) or glutathione (GSH) in mixed disulfide linkage with proteins. MAB3194 is specific to the glutathione:N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide. For use in ELISA or flow cytometry, sample must first be reacted with NEM.
      IsotypeIgG1
      Species Reactivity
      • Mammals
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3194 04053252265754

      Documentation

      Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT 2472914
      MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) MONOCLONAL ANTIBODY - 2383488 2383488
      MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 2705084 2705084
      MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3070206 3070206
      MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3185616 3185616
      MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3463581 3463581
      MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3613045 3613045
      MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3729360 3729360
      MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) - 3998964 3998964
      MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM) -2545378 2545378
      Show more

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research

      Data Sheet

      Title
      MOUSE ANTI-GLUTATHIONE: N-ETHYLMALEIMIDE ADDUCT (GS-NEM)