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07-1208 Anti-Gβ5 Antibody

This Anti-Gβ5 Antibody is validated for use in WB for the detection of Gβ5.

Anti-Gβ5 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: GPCR, cAMP/cGMP & Calcium Signaling Gene Symbol: GNB5, FLJ37457, FLJ43714, Gbeta5, GB5 UniProt Number: O14775
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07-1208
100 µg  
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      Overview

      Replacement Information
      Replacement Information07-1208 is a recommended replacement for AB4068

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, HWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1208
      ReplacesAB4068
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Gβ5 Antibody
      Background InformationThe Gβ5 (GNB5 guanine nucleotide binding protein (G protein), beta 5) is a G protein subunit present predominantly in the central nervous system. This protein appears to be expressed in two forms, one found in the brain of 39 kDa and one found in the retina of 44 kDa. It has also been found that Gβ5 associates tightly with RGS7, a member of the superfamily of negative regulators of G protein signaling. These complexes have been localized to most regions of the brain, whereas G1-4 exhibit no observable colocalization with RGS7, despite their widespread codistribution throughout similar neuronal networks. This antibody is specific for a peptide sequence within the D1 blade which is not found in Gβ1-4.
      References
      Product Information
      FormatPurified
      Control
      • L6 Cell Lysate, plasma membranes from rat cerebrum.
      PresentationPurified rabbit polyclonal IgG in phosphate buffered saline with 0.08% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Gβ5 Antibody is validated for use in WB for the detection of Gβ5.
      Key Applications
      • Western Blotting
      Application NotesOptimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenUnique peptide sequence derived from the D1 blade of the Gβ5 protein.
      EpitopeD1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes D1 area of Gβ5.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHeterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist.
      Gene Symbol
      • GNB5
      • FLJ37457
      • FLJ43714
      • Gbeta5
      • GB5
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O14775 # Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.
      SIZE: 395 amino acids; 43566 Da
      SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP (By similarity).
      TISSUE SPECIFICITY: Expressed in multiple tissues.
      SIMILARITY: SwissProt: O14775 ## Belongs to the WD repeat G protein beta family. & Contains 7 WD repeats.
      Molecular WeightThis protein appears to be expressed in two forms, one found in the brain of 39 kDa and one found in the retina of 44 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on L6 lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected G protein Gbeta5 on 10 μg of L6 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-1208 04053252370786

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies