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05-118 Anti-FGF-2/basic FGF Antibody, clone bFM-2

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05-118
500 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, M, RIHC, RIA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-118
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-FGF-2/basic FGF Antibody, clone bFM-2
      OverviewThis antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity. Upstate recommends anti-FGF-2/basic FGF, neutralizing (Catalog #05-117) for neutralizing activity.
      Alternate Names
      • Basic fibroblast growth factor
      • basic fibroblast growth factor bFGF
      • fibroblast growth factor 2
      • fibroblast growth factor 2 (basic)
      • heparin-binding growth factor 2
      Background InformationFGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
      Product Information
      FormatPurified
      Control
      • Fetal liver or kidney tissue.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS, pH 7.4, purified by ammonium sulfate precipitation and DEAE Sephacryl chromatography. Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationDetect FGF-2/basic FGF using this Anti-FGF-2/basic FGF Antibody, clone bFM-2 validated for use in IH, RIA & WB.
      Key Applications
      • Immunohistochemistry
      • Radioimmunoassay
      • Western Blotting
      Application NotesImmunohistochemistry:
      Previous lots of this antibody have been shown to immunostain frozen brain sections fixed with ice-cold, ethanol:acetic acid [95:5]. This antibody has also been used at 10 μg/mL to stain paraffin-embedded sections (Matsuzaki, K., 1989).
      Biological Information
      ImmunogenPurified bovine brain basic FGF (FGF-2, HBGF-2). Clone bFM-2.
      ClonebFM-2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody detects FGF-2/basic FGF.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Bovine
      • Mouse
      • Rat
      Species Reactivity NoteThis antibody is highly specific for basic FGF from bovine, human, rat, and mouse sources and does not cross-react with bovine acidic FGF (FGF-1, HBGF-1).

      Important Note: This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity (Uteza, Y., 1999). Upstate recommends anti-FGF-2/basic FGF, clone bFM-1 (neutralizing) (Catalog # 05-117) for neutralizing activity.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
      Gene Symbol
      • FGF2
      • Prostatropin
      • BFGF
      • prostatropin
      • HBGF-2
      • FGFB
      Purification MethodAmmonium sulfate precipitation and DEAE-cellulose chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09038 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
      SIZE: 155 amino acids; 17254 Da
      SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2.
      SIMILARITY: SwissProt: P09038 ## Belongs to the heparin-binding growth factors family.
      MISCELLANEOUS: This protein binds heparin more strongly than does aFGF.
      Molecular Weight17.5 kDa
      Product Usage Statements
      Quality AssuranceEvaluated on basic FGF (Catalog #01-114).

      Western Blot Analysis:
      0.5-2 μg/mL of this antibody detected 100 ng FGF-2/basic FGF, carrier-free (Catalog # 01-114).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size500 µg
      Global Trade Item Number
      Catalogue Number GTIN
      05-118 04053252361647