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07-689-25UL Anti-EZH2 (rabbit polyclonal)

This Anti-EZH2 Antibody is validated for use in Immunoprecipitation and Western Blotting and ChIP-seq for the detection of EZH2.

Anti-EZH2 (rabbit polyclonal) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: EZH2, MGC9169, ENX-1 UniProt Number: Q15910
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07-689-25UL
25 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WB, ChIP-seqRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-689-25UL
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-EZH2 (rabbit polyclonal)
      Alternate Names
      • KMT6
      • Enx1
      • Enhancer of Zeste Homologue 2
      Background InformationPolycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2) is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro.
      References
      Product Information
      FormatPurified
      Control
      • K562 cell lysate, C2C12 cell lysate
      PresentationPurified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-EZH2 Antibody is validated for use in Immunoprecipitation and Western Blotting and ChIP-seq for the detection of EZH2.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • ChIP-seq
      Biological Information
      ImmunogenHis-tagged fusion protein corresponding to residues 1-343 of mouse enhancer of Zeste homolog 2 (EZH2)
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityEZH2
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.
      Gene Symbol
      • EZH2
      • MGC9169
      • ENX-1
      Purification MethodAntiserum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15910 # May be involved in the regulation of gene transcription and chromatin structure.
      SIZE: 746 amino acids; 85363 Da
      SUBUNIT: Binds ATRX via the SET domain (Probable).
      SUBCELLULAR LOCATION: Nucleus (Probable).
      TISSUE SPECIFICITY: Expressed in many tissues.
      SIMILARITY: SwissProt: Q15910 ## Belongs to the EZ family. & Contains 1 SET domain.
      Molecular Weight95-100kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from HeLa and C2C12 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditionsstable for 2 years at 2-8°C from date of shipment
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-689-25UL 04054839350474