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ABN1653 Anti-Dysbindin

Anti-Dysbindin, Cat. No. ABN1653, is a highly specific rabbit polyclonal antibody that targets Dysbindin-1 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunoprecipitation, and Western Blotting.

Anti-Dysbindin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: DTNBP1, BLOC1S8, My031 UniProt Number: Q96EV8
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ABN1653
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, HIH(P), ICC, WB, IF, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1653
      ReplacesAB15450
      DescriptionAnti-Dysbindin
      Alternate Names
      • Biogenesis of lysosome-related organelles complex 1 subunit 8
      • BLOC-1 subunit 8
      • Dysbindin-1
      • Dystrobrevin-binding protein 1
      • Hermansky-Pudlak syndrome 7 protein
      • HPS7 protein
      Background InformationDysbindin (UniProt: Q96EV8; also known as Biogenesis of lysosome-related organelles complex 1 subunit 8, BLOC-1 subunit 8, Dysbindin-1, Dystrobrevin-binding protein 1, Hermansky-Pudlak syndrome 7 protein, HPS7 protein) is encoded by the DTNBP1 (also known as BLOC1S8, My031) gene (Gene ID: 84062) in human. Dysbindin is a component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. It plays a role in synaptic vesicle trafficking and in neurotransmitter release. Three isoforms produced by alternative splicing and alternative initiation have been described. Isoform 1 is mainly cytoplasmic, but shuttles between the cytoplasm and nucleus. It is exported out of the nucleus via its nuclear export sequence (NES). Its nuclear localization is required for regulation of the expression of genes, such as SYN1. It is detected in neuron cell bodies, axons and dendrites and is mainly located to the postsynaptic density. Isoform 2 also shuttles between the cytoplasm and nucleus and is mainly expressed in the dendritic spine. It is predominantly a synaptic vesicle isoform but also highly expressed in the nucleus. Isoform 3 is exclusively cytoplasmic and is predominantly found in the postsynaptic density (PSD) with little association with synaptic vesicles. Mutations in DTNBP1 gene have been linked to Hermansky-Pudlak syndrome 7 (HPS7) that is characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. Defects in DTNBP1 are associated with susceptibility to schizophrenia.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 100 mM sodium phosphate buffer (pH 7.0), 20 mg/ml BSA with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Dysbindin, Cat. No. ABN1653, is a highly specific rabbit polyclonal antibody that targets Dysbindin-1 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Western Blotting
      • Immunofluorescence
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Dysbindin in human cerebral cortex, human colon, and human kidney tissue.

      Immunohistochemistry Analysis: A representative lot detected Dysbindin in Immunohistochemistry applications (Ito, H., et. al. (2010). Mol Psychiatry. 15(10):976-86).

      Immunofluorescence Analysis: A 1,000 dilution from a representative lot detected Dysbindin in P8 mouse brain hippocampus tissue (Courtesy of Dr Koh-ichi Nagata at Aichi Human Service Center).

      Immunoprecipitation Analysis: A representative lot detected Dysbindin in Immunoprecipitation applications (Ito, H., et. al. (2010). Mol Psychiatry. 15(10):976-86).

      Immunocytochemistry Analysis: A representative lot detected Dysbindin in Immunocytochemistry applications (Yuan, Q., et. al. (2016). Biol Psychiatry. 80(4):312-22).

      Western Blotting Analysis: A representative lot detected Dysbindin in Western Blotting applications (Lee, S.A., et. al. (2015). J Biol Chem. 290(11):7087-96).

      Immunoprecipitation Analysis: A representative lot detected Dysbindin in Immunoprecipitation applications (Lee, S.A., et. al. (2015). J Biol Chem. 290(11):7087-96).

      Western Blotting Analysis: A representative lot detected Dysbindin in Western Blotting applications (Ito, H., et. al. (2010). Mol Psychiatry. 15(10):976-86).

      Immunocytochemistry Analysis: A representative lot detected Dysbindin in Immunocytochemistry applications (Ito, H., et. al. (2010). Mol Psychiatry. 15(10):976-86).
      Biological Information
      ImmunogenMBP-tagged full length recombinant human Dysbindin.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects Dysbindin in various regions of human, mouse, and rat brain.
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DTNBP1
      • BLOC1S8
      • My031
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight39.49 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human cerebellum tissue.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Dysbindin in human cerebellum tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1653 04054839193583