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ABS1644 Anti-DsbA-L

Anti-DsbA-L, Cat. No. ABS1644, is a rabbit polyclonal antibody that detects Glutathione S-transferase kappa 1 (DsbA-L) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

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ABS1644
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS1644
      DescriptionAnti-DsbA-L
      Alternate Names
      • Glutathione S-transferase kappa 1
      • EC:2.5.1.18
      • GST 13-13
      • GST class-kappa
      • GSTK1-1
      • mGSTK1
      • Glutathione S-transferase subunit 13
      Background InformationGlutathione S-transferase kappa 1 (UniProt: Q9DCM2; also known as EC:2.5.1.18, GST 13-13, GST class-kappa, GSTK1-1, mGSTK1, Glutathione S-transferase subunit 13, DsbA-L) is encoded by the Gstk1 gene (Gene ID: 76263) in murine species. DsbA-L is a disulfide bond A oxidoreductase like homodimeric mitochondrial protein that is predominantly expressed in heart, kidney, liver and skeletal muscle. It is also highly expressed in adipose tissue and its expression level is negatively correlated with obesity in mice and humans. Overexpression of DsbA-L is shown to promote adiponectin multimerization. Suppression of DsbA-L expression can selectively reduce adiponectin levels and secretion in 3T3-L1 adipocytes. Expression of DsbA-L in 3T3-L1 adipocytes is shown to be stimulated by the insulin sensitizer rosiglitazone and inhibited by TNFalpha. Adiponectin expression is reduced in both human obesity and mouse models of genetic obesity. DsbA-L is also shown to co-localize with the endoplasmic reticulum (ER) marker protein disulfide isomerase and it interacts with the ER chaperone protein Ero1-L in 3T3-L1 adipocytes. Deletion of the first 6 amino acid residues at the N terminus greatly impaired DsbA-L localization in the ER. (Ref.: Liu, M et al. (2015). J Biol Chem. 290(16):10143-8).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide with 20% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-DsbA-L, Cat. No. ABS1644, is a rabbit polyclonal antibody that detects Glutathione S-transferase kappa 1 (DsbA-L) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected DsbA-L in A431, HUVEC, NIH/3T3 cells.

      Western Blotting Analysis: A representative lot detected DsbA-L in mouse brain, mouse kidney, and mouse liver (Liu, M., et. al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).

      Immunoprecipitation Analysis: A representative lot detected DsbA-L in 3T3-L1 adipocytes (Liu, M., et. al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).

      Immunocytochemistry Analysis: A representative lot detected DsbA-L in adipocytes on differentiation day 7 were used for the following studies. confocal immunofluorescence study of DsbA-L co-localization with the mitochondrial marker (MitoTracker) and the ER marker (PDI) in 3T3-L1 adipocytes (Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).
      Biological Information
      ImmunogenMyc-tagged full length recombinant protein from mouse.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects DsbA-L (Glutathione S-transferase kappa 1) in human and mouse cells.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gstk1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~28 kDa observed; 25.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse kidney tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DsbA-L in 10 µg of mouse kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1644 04054839250958

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