Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
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Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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204869
Sigma-AldrichAnti-Complement 3 Goat pAb
This Anti-Complement 3 Goat pAb is validated for use in Flow Cytometry, Immunoblotting, Immunocytochemistry, Immunoelectrophoresis for the detection of Complement 3.
More>>This Anti-Complement 3 Goat pAb is validated for use in Flow Cytometry, Immunoblotting, Immunocytochemistry, Immunoelectrophoresis for the detection of Complement 3. Less<<
Anti-Complement 3 Goat pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Flow Cytometry (see application references) Immunoblotting (see application references) Immunocytochemistry (see application references) Immunoelectrophoresis (use undiluted)
Application Comments
Monospecific to human C3 complement component. This antibody has also been reported to recognize the C3 fragments, C3a, C3b, and C3c. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
purified, human C3 complement component
Immunogen
Human
Host
Goat
Isotype
IgG
Species Reactivity
Baboon
Guinea Pig
Hamster
Horse
Human
Mouse
Rabbit
Rat
Antibody Type
Polyclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
23-July-2007 RFH
Application
Flow Cytometry (see application references) Immunoblotting (see application references) Immunocytochemistry (see application references) Immunoelectrophoresis (use undiluted)
Description
Goat polyclonal antibody supplied as defibrinated, delipidized, and adsorbed (as needed) serum. Recognizes the C3 complement component.
Background
The complement system provides innate defense against microbial infection and is a "complement" to antibody mediated immunity. The complement system consists of thirty five interacting plasma and membrane associated proteins which contribute to host-defense and initiate and amplify inflammation, even in the preimmune state where specific antibodies and lymphocytes are not available. In addition to the complement components themselves, this system also contains several soluble factors that prevent spontaneous complement activation from occurring in solution, as well as, several regulatory membrane associated proteins that protect host cells from accidental complement attack. Cleavage of the C3 component by either the classical pathway or the alternative pathway releases C3a and C3b. C3a appears to be important in many inflammatory responses while the C3b fragment covalently binds to the cell or bacterial surface and plays a role in opsonisation. Binding of C3b to the C4b component of the C3 convertase, results in C5 convertase (C4b3b2a) formation.
Host
Goat
Immunogen species
Human
Immunogen
purified, human C3 complement component
Isotype
IgG
Species
baboon, not bovine, not canine, not chicken, not feline, guinea pig, hamster, horse, human, mouse, rabbit, rat, not sheep
Positive control
Purified C3 protein (Cat. No. 204885)
Form
Liquid
Formulation
Serum diluted in 500 mM NaCl, 50 mM Tris-HCl, pH 7.5.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
Monospecific to human C3 complement component. This antibody has also been reported to recognize the C3 fragments, C3a, C3b, and C3c. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Inal, J.M., et al. 2002. J. Immunol.168, 5213. Williams, B.J., et al. 2001. Infect. Immun.69, 695. Hindmarsh, E.J., et al. 1998. J. Immunol.160, 6128.