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AB144P-200UL Anti-Choline Acetyltransferase Antibody

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AB144P-200UL
200 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H, Ch, Av, Gp, OpICC, IHC, WBGtAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB144P-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Choline Acetyltransferase Antibody
      Alternate Names
      • Choline acetylase
      • acetyl CoA:choline O-acetyltransferase
      • choline acetyltransferase
      Background InformationAcetylcholine (ACh) is a common neuro-transmitter for motoneurons, preganglionic autonomic neurons, postganglionic para-sympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.
      Product Information
      FormatAffinity Purified
      Control
      • Rat hypothalamus tissue.
      PresentationPurified goat polyclonal in buffer containing PBS containing 5 mg/mL BSA and 0.2% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Choline Acetyltransferase (ChAT) Antibody is validated for use in IC, IH, WB for the detection of Choline Acetyltransferase.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was used in immunohistochemistry at 1:100 dilution. See attached protocol.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman placental enzyme.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGoat
      SpecificityCholine acetyltransferase found in cholinergic neurons in brain and central nervous systems.
      Species Reactivity
      • Mouse
      • Rat
      • Human
      • Chicken
      • Avian
      • Guinea Pig
      • Opossum
      Species Reactivity NoteRat, mouse, monkey, guinea pig, chicken, opossum, avian and human (western blot).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq].

      Transcript Variant: This variant (M) contains alternate 5' exon M, uses an upstream start codon, and encodes isoform 2.
      Gene Symbol
      • CHAT
      • ChAT
      • CHOACTase
      • CMS1A
      • CMS1A2
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
      SIZE: 748 amino acids; 82568 Da
      DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
      SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
      Molecular Weight70 kDa
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on NIH/3T3 lysates.

      Western Blot Analysis:
      1:1000 dilution of this antibody detected ChAT on 10 µg of NIH/3T3 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt.
      For short term storage (3-4 weeks) maintain at +2-8°C.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Global Trade Item Number
      Catalogue Number GTIN
      AB144P-200UL 04053252268984