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MABT1547-25UL Anti-CFAP206 Antibody, clone ORF2-2A7

Anti-CFAP206, clone ORF2-2A7, Cat. No. MABT1547, is a rat monoclonal antibody that detects CFAP206 and is tested for use in Western Blotting.

MABT1547-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1547-25UL
      DescriptionAnti-CFAP206 Antibody, clone ORF2-2A7
      Alternate Names
      • Cilia- and flagella-associated protein 206
      Background InformationCilia- and flagella-associated protein 206 (UniProt: Q6PE87; also known as CFAP206) is encoded by the Cfap206 gene (Gene ID: 69329) in murine species. CFAP206 is an evolutionarily conserved protein that lacks known motifs or domains, except for a unique 280 amino acid domain with conserved motifs GFC and GIL. It localizes to the basal body/ centrosome and to cilia, and its expression correlates with and depends on Foxj1 expression. In vertebrates it plays a role during motile cilia assembly, and its deficiency can lead to abnormal ciliary beating and decreased sperm motility. It is reported to be a potential component of the nexin-dynein regulatory complex and interacts with radial spoke protein 3. Two isoforms of CFAP206 have been described that are produced by alternative spicing. Isoform 1 is expressed in the sperm, oviduct, lung, nasal cavity, brain ependyma, and choroid plexus. This isoform is shown to be essential for sperm motility and is involved in the regulation of the beating frequency of motile cilia on the epithelial cells of the respiratory tract and it is required for the establishment of radial spokes in sperm flagella. Isoform 2 that is expressed in the sperm, oviduct, lung, nasal cavity, brain ependyma, and choroid plexus is also required for sperm motility and is involved in the regulation of the beating frequency of motile cilia on the epithelial cells of the respiratory tract. Clone ORF2-2A7 specifically detects isoform 1 and detects a sequence within 13 amino acids from the C-terminal region, which is missing in isoform 2. (Ref.: Beckers, A., et al. (2020). Development. 147(21); dev188052).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CFAP206, clone ORF2-2A7, Cat. No. MABT1547, is a rat monoclonal antibody that detects CFAP206 and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesTested applications

      Western Blotting Analysis: A representative lot detected CFAP206 in Western Blotting application. (Beckers, A., et al. (2020). Development. 147(21):dev188052).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenA linear peptide corresponding to 13 amino acids from the C-terminal region of mouse Cilia- and flagella-associated protein 206 (CFAP206).
      EpitopeC-terminal
      CloneORF2-2A7
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone ORF2-2A7 is a rat monoclonal antibody that detects Cilia- and flagella-associated protein 206 (CFAP206). It targets an epitope within 13 amino acids from the C-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cfap206
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~70 kDa observed; 70.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse testis tissue extract.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected CFAP206 in Mouse testis tissue extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1547-25UL 04065269366829