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MABN778 Anti-C9ORF72/C9RANT (poly-GR) Antibody, clone 5A2

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MABN778
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN778
      DescriptionAnti-C9ORF72/C9RANT (poly-GR) Antibody, clone 5A2
      Alternate Names
      • c9orf72
      Background InformationHexanucleotide (GGGGCC) repeat expansions in a noncoding region of C9ORF72 are the major genetic cause of FTD and ALS (c9FTD/ALS). The RNA structure of GGGGCC repeats renders these transcripts susceptible to an unconventional mechanism of translation—repeat associated non-ATG (RAN) translation. Translation of the GGGGCC-repeat in all reading frames would result in three dipeptide-repeat (DPR) proteins poly-(Gly-Ala), poly-(Gly-Pro) and poly-(Gly-Arg). poly-GA and poly-GP proteins are extremely hydrophobic and may form intracellular aggregates
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect C9ORF72/C9RANT (poly-GR) using this Anti-C9ORF72/C9RANT (poly-GR) antibody, clone 5A2 validated for use in western blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A representative lot detected poly-GR inclusions from brain tissues of C9orf72 patients with C9ORF72 hexanucleotide expansion (Mori, K., et al. (2013). Science. 339(6125):1335-1338.).
      Biological Information
      ImmunogenLinear peptide corresponding to Epstein-Barr Virus Nuclear Antigen 2 (ENBA2) containing GR repeat sequence.
      Epitopepoly-GR
      Clone5A2
      Concentration1 mg/mL
      HostRat
      SpecificityThis antibody recognizes C9ORF72/C9RANT (poly-GR) and other proteins containing poly-GR sequence, such as the Epstein Barr virus protein EBNA2
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with all species.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Molecular WeightVariable
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting using GST® fusion proteins with 15 GA, GP, or GR repeats, as well as GST alone without any repeat sequence.

      Western Blotting Analysis: 0.2 μg of this antibody detected 10 μg of GR-GST recombinant protein with 15 GR repeats.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MABN778 04053252947063