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05-449-C Anti-Bin1, clone 99D (Ascites Free) Antibody

This Anti-Bin1, clone 99D (Ascites Free) Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation and Flow Cytometry and Immunofluorescence for the detection of Bin1.

Anti-Bin1, clone 99D (Ascites Free) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: BIN1, AMPHL UniProt Number: O00499
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05-449-C
100 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ch, MWB, IHC, IP, FC, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-449-C
      DescriptionAnti-Bin1, clone 99D (Ascites Free) Antibody
      Alternate Names
      • Myc box-dependent-interacting protein 1
      • Amphiphysin II
      • Amphiphysin-like protein
      • Box-dependent myc-interacting protein 1
      • Bridging integrator 1
      • Bin1
      Background InformationBin1, also known as Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, and encoded by the gene BIN1/AMPHL, is another protein involved in the regulation of synaptic vesicle endocytosis and interestingly also acts as tumor suppressor and inhibits malignant cell transformation. BIN1 is expressed in many tissues but has the highest expression in brain and muscle. BIN1 has isoforms and particular isoforms are expressed in particular tissues. For instance BIN1 isoform 11A is only expressed in the brain and found in axon initial segments and nodes of Ranvier. Mutations in BIN1 are associated with a disordered called centronuclear 2 myopathy. BIN1 is localized to nucleus but some isoforms are associated with cytoplasm.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Bin1, clone 99D (Ascites Free) Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation and Flow Cytometry and Immunofluorescence for the detection of Bin1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      • Flow Cytometry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Bin1 in human cerebellum and human skeletal muscle myocytes tissue.
      Western Blotting Analysis: A representative lot detected Bin1 in primary chicken embryo fibroblasts (Telfer, J.F., et al. (2005). Cellular Signalling. 17:701-8).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
      Western Blotting Analysis: A representative lot detected Bin1 in human and rodent cell lines (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in co-immunoprecipitation studies (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
      Immunohistochemistry Analysis: A representative lot detected Bin1 in breast and liver carcinoma tissues (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
      Western Blotting Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
      Flow Cytometry Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
      Immunofluorescence Analysis: A representative lot detected Bin1 in C2C12 cells (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
      Biological Information
      ImmunogenLinear peptide corresponding to human Bin1.
      Clone99D
      Concentration1 mg/mL
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Chicken
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BIN1
      • AMPHL
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~60 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human skeletal muscle tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Bin1 in 10 µg of human skeletal muscle tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-449-C 04053252978746

      Documentation

      Anti-Bin1, clone 99D (Ascites Free) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Bin1, clone 99D (Ascites Free) Antibody Certificates of Analysis

      TitleLot Number
      Anti-Bin1, clone 99D (Ascites Free) - Q2435642 Q2435642
      Anti-Bin1, clone 99D (Ascites Free) - 3299962 3299962
      Anti-Bin1, clone 99D (Ascites Free) - 3538142 3538142
      Anti-Bin1, clone 99D (Ascites Free) - 3956980 3956980
      Anti-Bin1, clone 99D (Ascites Free) -2707213 2707213
      Anti-Bin1, clone 99D (Ascites Free) Monoclonal Antibody 3137648
      Anti-Bin1, clone 99D (Ascites Free) Monoclonal Antibody 2979478

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies