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MABC1638-100UL Anti-AIFM2 Antibody, clone 6D8-11

Anti-AIFM2, clone 6D8-11, Cat. No. MABC1638, is a rat monoclonal antibody that detects Ferroptosis suppressor protein 1 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting.

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MABC1638-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1638-100UL
      DescriptionAnti-AIFM2 Antibody, clone 6D8-11
      Alternate Names
      • Ferroptosis suppressor protein 1
      • FSP1
      • Apoptosis-inducing factor homologous mitochondrion-associated inducer of death
      • AMID
      • p53-responsive gene 3 protein
      Background InformationFerroptosis suppressor protein 1 (UniProt: Q9BRQ8; also known as FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, AMID, p53-responsive gene 3 protein, AIFM2) is encoded by the AIFM2 (also known as AMID, PRG3) gene (Gene ID: 84883) in human. AIFM2 is a lipid-anchor membrane protein of the FAD-dependent oxidoreductase family that is also detected in the nucleus and mitochondrial membrane. It is highly expressed in heart and moderately in liver and skeletal muscle. Low expression levels are observed in placenta, lung, kidney, and pancreas. It is reported to be down-regulated in a number of human tumors. It serves as a NAD(P)H-dependent oxidoreductase and is involved in cellular oxidative stress response. At the plasma membrane, it catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. It is shown to cooperates with GPX4 to suppress phospholipid peroxidation and ferroptosis and its anti-ferroptotic function is reported to be independent of cellular glutathione levels. It also plays a role in mitochondrial stress signaling. Upon induction of oxidative stress, it associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct. This HNE adduct inactivates the NADH oxidoreductase activity of AIFM2 and facilitates its translocation from mitochondria into the nucleus triggering DNA damage and cell death. (Ref.: Doll, S., et al. (2019). Nature 575(7784); 693-698; Miriyala, S., et al. (2016). Free Radic. Biol. Med. 91; 68-80).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-AIFM2, clone 6D8-11, Cat. No. MABC1638, is a rat monoclonal antibody that detects Ferroptosis suppressor protein 1 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunofluorescence
      • ELISA
      Application NotesTested Appliacations

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected AIFM2 in HepG2 cell lysate.

      Immunocytochemistry Analysis: A 1:25 Dilution from a representative lot detected AIFM2 in HepG2 cells.

      Immunofluorescence Analysis: A representative lot detected AIFM2 in Immunofluorescence applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).

      Western Blotting Analysis: A representative lot detected AIFM2 in Western Blotting applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).

      ELISA Analysis: A representative lot detected AIFM2 in ELISA applications (Doll, S. et al. (2019). Nature.;575(7784):693-698).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenSUMO-His-tagged full-length recombinant human Ferroptosis suppressor protein 1 (FSP1/AIFM2).
      EpitopeUnknown
      Clone6D8
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 6D8 is a rat monoclonal antibody that detects human Ferroptosis suppressor protein 1 (AIFM2).
      IsotypeIgG2a
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AIFM2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~40 kDa observed; 40.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected AIFM2 in A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1638-100UL 04065266908190