Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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194933
Sigma-AldrichAZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem
AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem Certificates of Analysis
Title
Lot Number
194933
References
Reference overview
Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157. Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
31-October-2016 JSW
Description
General proteolytic substrate. Smaller particle size provides a rapid, simple method for quantitative determination of protease content in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 5 mg/ml buffer, 10 min at 37°C, pH 7.0): <0.3. Mesh: >100.
Form
Dark red solid
Recommended reaction conditions
Assay Protocol for Protease Activity
1. Dissolve 50 mg AZOCOLL® in 5 ml of buffer solution (e.g., PBS, pH 7.0)
containing the proteolytic enzyme.
2. Incubate in stoppered tubes for 15 min at 37°C, agitating gently at 5 min intervals.
3. Cool the tubes in ice water and filter the contents through Whatman No. 1 filter paper.
4. Measure the absorbance at 520 nm, subtracting the blank value.
5. Plot absorbance versus protease activity. A linear relationship exists between protease concentration and the rate of dye liberation.
Note: The rate of color yield from AZOCOLL® will vary with the individual proteases tested. This protocol is meant to serve as a guideline and may need to be modified for specific applications.
CAS number
53092-90-3
Solubility
PBS (10 mg/ml)
Storage
Protect from light +15°C to +30°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157. Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.