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506114 p34cdk1/Cyclin B, Human, Recombinant, S. frugiperda

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506114
Preis & Verfügbarkeit

Übersicht

Replacement Information

Preis & Verfügbarkeit

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506114-200U
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      Description
      OverviewRecombinant, human p34Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, Cdk1 is inactive and requires association with cyclin B and phosphorylation of Thr161 to form an active complex. MW: 34000(p34Cdk1) and 55000(cyclinB).
      Catalogue Number506114
      Brand Family Calbiochem®
      References
      ReferencesKobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
      Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.
      Product Information
      Activity≥20,000 U/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      ContaminantsProtease, phosphatase: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      506114-200U 04055977199444

      Documentation

      p34cdk1/Cyclin B, Human, Recombinant, S. frugiperda SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      p34cdk1/Cyclin B, Human, Recombinant, S. frugiperda Analysenzertifikate

      TitelChargennummer
      506114

      Literatur

      Übersicht
      Kobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
      Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2008 RFH
      DescriptionRecombinant, human p34Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, p34cdc2 is inactive and requires association with cyclin B to form an active complex.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
      Recommended reaction conditions50 mM Tris-HCl, 10 mM MgCl2, 2 mM DTT, 1 mM EGTA, 100 µM ATP, 0.01% BRIJ® 35 detergent, pH 7.5.
      ContaminantsProtease, phosphatase: none detected
      Activity≥20,000 U/ml
      Unit definitionOne unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesKobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
      Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.