Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
SCC627
Sigma-AldrichmEERL Mouse E6/E7/hRas Oropharynx Epithelial Luciferase Cell Line
The SCC627 mEERL Mouse E6/E7/hRas Oropharynx Epithelial Luciferase Cell Line expresses luciferase and is useful in the study of head and neck squamous cell carcinoma in an immunocompetent, syngeneic model.
More>>The SCC627 mEERL Mouse E6/E7/hRas Oropharynx Epithelial Luciferase Cell Line expresses luciferase and is useful in the study of head and neck squamous cell carcinoma in an immunocompetent, syngeneic model. Less<<
Empfohlene Produkte
Übersicht
Replacement Information
Description
Catalogue Number
SCC627
Description
mEERL Mouse E6/E7/hRas Oropharynx Epithelial Luciferase Cell Line
Overview
SCC627, the luciferase-expressing derivative of SCC626, retains the capability for invasive growth, as well as the expression of many of the hallmark genes of OPSCC. These include cytokeratin, E-cadherin, BRCA2, p16, and EGFR. SCC627 was successfully used to optically track metastasis through the lymphatic space to the lungs, which is anatomically relevant to human oropharyngeal cancers. Along with SCC625 and SCC626, this series of cell lines comprise a suitable platform for mechanistic studies of OPSCC and for testing therapeutic intervention strategies.
Alternate Names
mEERL Luciferase Cell Line
Mouse E6/E7/hRas Luciferase Cell Line
HPV E6/E7/hRas Luceiferase Cell Line
OPSCC Luciferase Cell Line
HNSCC Luciferase Cell Line
Background Information
The head and neck cancers represent the sixth most common class of cancer. Among them, incidents of oropharyngeal squamous cell carcinoma (OPSCC) are on the rise, and a majority of them are caused by the human papilloma virus (HPV). Although successful management methods exist, local recurrence and nodal metastasis continue to result in mortality in certain cases. Historically, insights into HPV-induced transformation have been obtained in the context of the cervical cancer and anogenital warts models. While much of it is relevant to OPSCC, it will be beneficial to have the systems in which the effects of HPV infection can be studied in a context specific to OPSCC. The parental strain for SCC627, the mouse E6/E7/hRas/luciferase (mEERL) cell line, was established by the retroviral transduction of the HPV E6 gene, the HPV E7 gene, and the human hRas G12V gene into the primary mouse oropharyngeal epithelial cells. Due to the synergistic effects of the three oncogenes, the resulting cell line (also available as SCC626, the mEER cell line) was shown to be capable of cultivation, anchorage-independent growth in vitro, and the formation of poorly-differentiated, aggressively metastatic tumors following orthotopic injection into immune-competent mice.
SOURCE
SCC627 is a luciferase-expressing derivative of the mEER cell line (SCC626). SCC626 was established by the retroviral transduction of the HPV16 E6 gene, HPV16 E7 gene, and the human hRas gene into the primary oropharyngeal epithelial cells isolated from a male C57BL/6 mouse.
REFERENCES
1. Kademani D. 2007. Oral cancer. Mayo Clin Proc. 82(7): 878-87. 2. Rogers SN, Brown JS, Woolgar JA, Lowe D, Magennis P, Shaw RJ, Sutton D, Errington D, Vaughan D. 2009. Survival following primary surgery for oral cancer. Oral Oncol. 45(3):201-11. 3. Pytynia KB, Dahlstrom KR, Sturgis EM. 2014. Epidemiology of HPV-associated oropharyngeal cancer. Oral Oncol. 50(5): 380-6. 4. Hoover AC, Spanos WC, Harris GF, Anderson ME, Klingelhutz AJ, Lee JH. 2007. The role of human papillomavirus 16 E6 in anchorage-independent and invasive growth of mouse tonsil epithelium. Arch Otolaryngol Head Neck Surg. 133(5): 495-502. 5. Williams R, Lee DW, Elzey BD, Anderson ME, Hostager BS, Lee JH. 2009. Preclinical models of HPV+ and HPV- HNSCC in mice: an immune clearance of HPV+ HNSCC. Head Neck. 31(7): 911-8. 6. Vermeer DW, Coppock JD, Zeng E, Lee KM, Spanos WC, Onken MD, Uppaluri R, Lee JH, Vermeer PD. 2016. Metastatic model of HPV+ oropharyngeal squamous cell carcinoma demonstrates heterogeneity in tumor metastasis. Oncotarget. 7(17): 24194-207.
References
Product Information
Components
≥1x106 viable mEERL Mouse E6/E7/hRas Oropharynx Epithelial Luciferase Cells: (Catalog No. SCC627). Store in liquid nitrogen.
The SCC627 mEERL Mouse E6/E7/hRas Oropharynx Epithelial Luciferase Cell Line expresses luciferase and is useful in the study of head and neck squamous cell carcinoma in an immunocompetent, syngeneic model.
Key Applications
Cell Culture
Application Notes
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
Biological Information
Host
Mouse
Cell Line Type
Cancer Cells
Epithelial Cells
Luciferase assay
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
mEERL cells are verified to be of mouse origin and negative for human, rat, Chinese hamster, Golden Syrian hamster, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services Cells tested negative for infectious diseases against a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services. Cells tested negative for mycoplasma.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Storage and Shipping Information
Storage Conditions
mEERL cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size
>= 1 x 10^6 viable cells per vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Bestellnummer
GTIN
SCC627
04065270977410
Documentation
mEERL Mouse E6/E7/hRas Oropharynx Epithelial Luciferase Cell Line SDB