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48-602MAG
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The Wnt5a protein is a 352 amino acid protein containing 23 conserved cysteines & contains a cysteine modified by palmitate which is essential for receptor binding & biological activity.
More>>The Wnt5a protein is a 352 amino acid protein containing 23 conserved cysteines & contains a cysteine modified by palmitate which is essential for receptor binding & biological activity. Less<<
Wnt-5a Protein, Recombinant mouse: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Wnt5a has been purified from conditioned media using blue-Sepharose™, gel filtration and heparin affinity chromatography.
Not determined. Endotoxin level is less than 0.1 ng per μg of Wnt5a.
Description
Catalogue Number
GF146
Brand Family
Chemicon®
Trade Name
Chemicon
Description
Wnt-5a Protein, Recombinant mouse
Overview
Wnt5a, a member of the highly conserved Wnt protein family, is a 352 amino acid protein containing 23 conserved cysteines and contains a cysteine modified by palmitate which is essential for receptor binding and biological activity. The Wnt5a amino acid sequence also contains an N-terminal signal peptide which means the protein is secreted into the medium when expressed in cell culture.
Wnt ligands bind to cell surface co-receptors consisting of a Frizzled protein and lipoprotein receptor related protein (5/6). Wnt 5a belongs to the non-canonical class of Wnt proteins which are independent of or inhibit β-catenin signalling. Wnt5a when bound to its receptor triggers an intracellular Ca release which activates PKC and CaMK11 which in turn activates TAK1 MAPkinase-kinase and nemo-like(NLK) kinase. NLK phosphorylates Lef1/TCF protein complex inhibiting the formation of the β-catenin Lef1/TCF DNA complex. In contrast Wnt3a signalling stabilizes β-catenin increasing the formation of the β-catenin Lef1/TCF DNA complex.
Wnt signalling has been implicated in the control of differentiation of stem cells. The Wnts have also been shown to have putative roles in the regulation of adult stem cells. Recently injection of embryonic stem cells into hearts has been shown to correct cardiac defects. This occurs through the secretion of factors from stem cells which correct gene defects in neighbouring cells. Wnt5a, a short term factor secreted by the stem cells, has been shown to be critical for this process (Fraidenraich and Benzra, 2006).
The Wnt5a protein is a 352 amino acid protein containing 23 conserved cysteines & contains a cysteine modified by palmitate which is essential for receptor binding & biological activity.
Key Applications
Cell Culture
Application Notes
• This protein is extremely sticky and will stick to plasticware in the absence of Chaps detergent. • When using this protein, if possible, dose it directly onto cells at the required dilution without pre-filtering. • The toxicity of the Chaps in the storage buffer should be tested empirically on the cell line prior to using the Wnt5a protein. • Generally, we suggest that a minimum dilution of Wnt5a of 1:1000 on mammalian cells results in a final concentration of Chaps that is not toxic to the cells. • If dosing on cells over a period of days/weeks, it is recommended that media containing Wnt5a is replenished daily, rather than dosing Wnt5a to existing media daily. This is because accumulation of Chaps is undesirable for cell viability.
Biological Information
Concentration
80-100 ng/μL
Purity
Not determined. Endotoxin level is less than 0.1 ng per μg of Wnt5a.
Source
Wnt5a has been purified from conditioned media using blue-Sepharose™, gel filtration and heparin affinity chromatography.
Specific Activity
The biological activity of Wnt5a has been demonstrated by its ability to antagonize the induction of alkaline phosphatase synthesis by Wnt3a in the presence of BMP4 by C2C12 osteoblastic cells. The EC50 for this effect is typically 100 ng/mL. <i>Optimal concentrations should be determined for each application.</i>
Entrez Gene Summary
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.
FUNCTION: SwissProt: P41221 # Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. SIZE: 365 amino acids; 40887 Da SUBUNIT: Interacts with PORCN (By similarity). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. SIMILARITY: SwissProt: P41221 ## Belongs to the Wnt family.
Stem Cell Type
Neural Stem Cells
Mesenchymal Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain the frozen material at -20 °C until expiration date as stated on the label. Upon receipt, thaw and aliquot into single-use aliquots and store at -20 °C. Once a single-use aliquot has been thawed, DO NOT re-freeze. Store thawed material at 4 °C for up to one month.
Embryonic stem cells prevent developmental cardiac defects in mice Fraidenraich, Diego and Benezra, Robert Nature clinical practice Cardiovascular medicine, 3 Suppl 1:S14-7 (2006)
2005
EMD Millipore offers a comprehensive range of highly purified bioactive cytokines and growth factors for use in human, mouse, and rat cell culture, stem cell differentiation and ELISA studies. Weitere Informationen >>
