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DR1002 PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12)

This PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) is validated for use in Immunoblotting, Immunocytochemistry for the detection of ATM (pSer¹⁹⁸¹).

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)

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DR1002
Preis & Verfügbarkeit

Übersicht

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Preis & Verfügbarkeit

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DR1002-50UG
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      Description
      Overview

      This product has been discontinued.



      Recognizes the ~370 kDa ATM protein phosphorylated on Ser1981 in camptothecin-treated HeLa cells. Sold under license of U.S. Patent 7,108,992.
      Catalogue NumberDR1002
      Brand Family Calbiochem®
      SynonymsAnti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)
      Application Data
      Detection of human ATM phosphorylated at Ser1981 by immunoblotting. Samples: Whole cell lysate (50 µg) from untreated HeLa cells (left lane) and camptothecin-treated (100 µM) HeLa cells (right lane). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (0.5 µg/ml). Detection: chemiluminescence.

      Detection of human ATM phosphorylated at (Ser1981) by immunofluorescence. Samples: Untreated HeLa cells (left panel) and camptothecin-treated (10 µM, 2 h) HeLa cells, fixed with 100% methanol (right panel). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (1.5 µg/ml). Detection: fluorescence.
      References
      ReferencesHorejsi, Z., et al. 2004. Oncogene 23, 3122.
      Abraham, R.T. 2003. Genes Dev. 15, 2177.
      Bakkenist, C.J. and Kastan, M.B. 2003. Nature 421, 499.
      Shiloh, Y. 2003. Nat. Rev. Cancer 3, 155.
      Product Information
      DeclarationSold under license of U.S. Patent 7,108,992.
      FormLiquid
      FormulationIn PBS.
      Positive controlHeLa cells treated with camptothecin (Cat. No. 208925)
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (0.5 µg/ml)
      Immunocytochemistry (1.5 µg/ml)
      Application CommentsDoes not detect the unphosphorylated protein. Use 5% milk in TBS-T for blocking; the use of BSA is not recommended. The use of PBS-T is not recommended, as phosphate-containing buffers can contribute to high background with phosphospecific antibodies. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser¹⁹⁸¹ phosphorylation site of human ATM
      ImmunogenHuman
      Clone10H11.E12
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      DR1002-50UG 04055977225891

      Documentation

      PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) Analysenzertifikate

      TitelChargennummer
      DR1002

      Literatur

      Übersicht
      Horejsi, Z., et al. 2004. Oncogene 23, 3122.
      Abraham, R.T. 2003. Genes Dev. 15, 2177.
      Bakkenist, C.J. and Kastan, M.B. 2003. Nature 421, 499.
      Shiloh, Y. 2003. Nat. Rev. Cancer 3, 155.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      SynonymsAnti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)
      ApplicationImmunoblotting (0.5 µg/ml)
      Immunocytochemistry (1.5 µg/ml)
      Application Data
      Detection of human ATM phosphorylated at Ser1981 by immunoblotting. Samples: Whole cell lysate (50 µg) from untreated HeLa cells (left lane) and camptothecin-treated (100 µM) HeLa cells (right lane). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (0.5 µg/ml). Detection: chemiluminescence.

      Detection of human ATM phosphorylated at (Ser1981) by immunofluorescence. Samples: Untreated HeLa cells (left panel) and camptothecin-treated (10 µM, 2 h) HeLa cells, fixed with 100% methanol (right panel). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (1.5 µg/ml). Detection: fluorescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~370 kDa ATM protein phosphorylated at Ser1981.
      BackgroundATM (ataxia telangiectasia mutated, ACC# NP_000042) is a member of the PI-3/PI-4 kinase family and is a central mediator of the cellular response to DNA damage and cellular stress. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. ATM is a regulator of numerous downstream proteins, including the tumor suppressor proteins, p53 and BRCA1, the checkpoint kinase, CHK2, the checkpoint proteins RAD17 and RAD9, and the DNA repair protein NBS1. ATM and the functionally related ATR kinase are thought to be master regulators of cell cycle checkpoint signaling pathways that are required for the cellular response to DNA damage and as well as genome stability. Activation of ATM in response to DNA damage requires intermolecular autophosphorylation of Ser1981 and dimer dissociation.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser¹⁹⁸¹ phosphorylation site of human ATM
      Clone10H11.E12
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlHeLa cells treated with camptothecin (Cat. No. 208925)
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not detect the unphosphorylated protein. Use 5% milk in TBS-T for blocking; the use of BSA is not recommended. The use of PBS-T is not recommended, as phosphate-containing buffers can contribute to high background with phosphospecific antibodies. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesHorejsi, Z., et al. 2004. Oncogene 23, 3122.
      Abraham, R.T. 2003. Genes Dev. 15, 2177.
      Bakkenist, C.J. and Kastan, M.B. 2003. Nature 421, 499.
      Shiloh, Y. 2003. Nat. Rev. Cancer 3, 155.