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48-624MAG MILLIPLEX® 2-Plex Phospho/Total p38 - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling Research Sub-Category: Neurodegenerative Diseases
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48-624MAG
96-well plate  96-well plate
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      Übersicht

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      p38 (Total) p38 (Thr180/Tyr182) H, M, RMplexLuminex xMAP
      Description
      Catalogue Number48-624MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX® 2-Plex Phospho/Total p38 - Cell Signaling Multiplex Assay
      OverviewThe MILLIPLEX® 2-Plex Phospho/Total p38 magnetic bead kit has been developed for the simultaneous detection of phosphorylated p38 (Thr180/Thr182) and total p38 in a single well using the Luminex® system. This detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.
      Background InformationProtein phosphorylation represents a key mechanism used in regulating cellular functions in all eukaryotic cells. Aberrant phosphorylation has been implicated in the onset and development of many diseases including metabolic disorders, inflammatory disease, cancer, etc. Changes in protein phosphorylation can be attributed to changes in both phosphorylation events, as well as changes due to total protein levels. To distinguish the changes in phosphorylation from changes in protein expression it is important to normalize the signal from phosphorylation over the signal from total protein. For this need, the MILLIPLEX® MAP 2-plex Phospho/Total p38 kit has been developed for the simultaneous detection of phosphorylated p38 (Thr180/Tyr182) and total p38 in a single well using the Luminex system. According to the original vendor’s antibody information sheets, phospho-p38 antibody pair detects p38α, p38β and p38γ but not p38-δ, while the total p38 antibody pair detects only p38α. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples. Each kit has sufficient reagents for one 96 well plate assay.
      References
      Product Information
      Components
      • MILLIPLEX® 2-plex Phospho/Total p38, Magnetic Beads (20X), 1 tube, 180 uL
      • MILLIPLEX® 2-plex Phospho/Total p38, Biotin (20X) Detection Antibody, 1 tube, 180 uL
      • MILLIPLEX® Lysis Buffer (1X), 1 bottle, 55 mL
      • MILLIPLEX® Assay Buffer 2 (1X), 1 bottle, 55 mL
      • MILLIPLEX® HeLa Cell Lysate: Unstimulated, 1 vial
      • MILLIPLEX® HeLa Cell Lysate: TNFa/Calyculin A, 1 vial
      • MILLIPLEX® Streptavidin-Phycoerythrin (25X), 1 tube, 150 uL
      • MILLIPLEX® Amplification Buffer (1X), 1 bottle, 3 mL
      • Empty mixing bottles, 3 bottles
      • 96 well Plate, 1 plate with 2 plate sealers
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Panel TypeCell Signaling
      Quality LevelMQ200
      Applications
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted, filtered cell lysate per well.
      • This kit must be run using the provided Assay Buffer.
      • The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL)
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Analytes Available
      • p38 (Total)
      • p38 (Thr180/Tyr182)
      Assay PanelEverything you need in a single kit.
      Physicochemical Information
      Sensitivity
      • Refer to kit protocol for sensitivities of individual biomarkers.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96-well plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      48-624MAG 04055977277791