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SCC613 LX-2 Cas9 Human Hepatic Stellate Cell Line

The LX-2 Cas9 human hepatic stellate cell line has been extensively characterized and retains key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis , making it a highly suitable model of human hepatic fibrosis.

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SCC613
≥1X10⁶ cells/vial   
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      Replacement Information
      Description
      Catalogue NumberSCC613
      DescriptionLX-2 Cas9 Human Hepatic Stellate Cell Line
      Alternate Names
      • HSC Cas9
      • Perisinusoidal cells Cas9
      • Ito cells Cas9
      • Hepatic pericytes Cas9
      • Hepatic stellate cells Cas9
      Background InformationHepatic stellate cells are a cell type responsible for liver fibrosis following their activation to fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. Given the importance of stellate cells in disease etiology, genetically malleable cellular models are critical for understanding the contribution of these cells to liver pathologies and necessary for development of new therapeutics.

      The immortalized LX-2 line was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.

      Source
      LX-2 Cas9 human hepatic stellate cells were derived from LX-2 cells transformed with a Cas9 cassette.
      LX-2 was generated by immortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passage cells in low serum media conditions.

      References
      1. Gut 2005, 54(1): 142-151.
      2. PloS One 2013, 8(10): e75692.
      References
      Product Information
      Components
      • ≥1x106 viable LX-2 Cas9 Human Hepatic Stellate Cells: (Catalog No. SCC613). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationThe LX-2 Cas9 human hepatic stellate cell line has been extensively characterized and retains key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis , making it a highly suitable model of human hepatic fibrosis.
      Key Applications
      • Cell Culture
      • Cell Function Assay
      • Function Assay
      • Toxicology Assays
      • Cell Based Assays
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Hepatocyte Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested negative for infectious diseases by a Human Essential CLEAR Panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of human origin and negative for interspecies contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsLX-2 Cas9 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and functionality. LX-2 cells have been successfully expanded beyond passage 50 in culture.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      SCC613 04065268102060