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IPVH304F0 Immobilon-P Membran, PVDF, 0,45 µm, 26 x 26 cm-Blätter

Western Blotting, Bindungsstudien, Aminosäure-Analysen, N-terminale Proteinsequenzierung, Dot/Slot Blotting, Glycoproteinbestimmung, Lipopolysaccharid-Analyse

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Hauptanwendungen: Western Blotting, Dot Blot
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IPVH304F0
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      Übersicht

      Replacement Information

      Key Spec Table

      Hauptanwendungen
      WB, DB
      Beschreibung
      BestellnummerIPVH304F0
      Markenname
      • Immobilon
      BeschreibungImmobilon-P Membran, PVDF, 0,45 µm, 26 x 26 cm-Blätter
      HintergrundinformationenOverview
      - Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
      Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

      Immobilon-P Transfer Membrane:
      - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
      - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

      Immobilon-E Membrane:
      - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
      - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

      Blotting Sandwiches:
      - When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

      Blotting Filter Paper:
      - Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

      Immobilon-PSQ Membrane:
      - 0.2 µm pore size and a large internal structure
      - Higher protein adsorption and sequencing yields than other membranes
      - Recommended for blotting proteins less than 20 kDa
      - Prevents blow-through of low molecular weight proteins

      Immobilon-FL Membrane:
      - The first transfer membrane optimized for fluorescence applications
      - Extremely low background improves sensitivity of all fluorescence detection protocols
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      - Ideal for multiplexing and chemifluorescence applications

      Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

      Features & Benefits:
      - Won’t crack, curl or fracture when cut
      - Low background
      - Superior staining capabilities
      - Can be reprobed multiple times

      Applications:
      - Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
      - Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      Literatur
      Produktinformationen
      NachweismethodeChemiluminesenz Chromogen Radioaktiv
      Kompatible Färbemittellösungen
      • Coomassie-Brilliant-Blue
      • Amidoschwarz
      • India-Ink
      • Ponceau-S-Rot
      • Kolloidales Gold
      • CPTS
      • Toluidinblau
      • Transillumination
      • Sypro®-Ruby-Farbstoff
      FiltercodeIPVH
      FilterfarbeWeiß
      Quality LevelMQ400
      Anwendungen
      AnwendungWestern Blotting, Bindungsstudien, Aminosäure-Analysen, N-terminale Proteinsequenzierung, Dot/Slot Blotting, Glycoproteinbestimmung, Lipopolysaccharid-Analyse
      Hauptanwendungen
      • Western Blotting
      • Dot Blot
      Biologische Informationen
      Primäre BindungsmechanismenElektrostatisch, hydrophob
      Rinderserumalbumin-Adsorption215 µg/cm²
      Ziegen-IgG-Adsorption294 µg/cm²
      Insulin-Adsorption160 µg/cm²
      BenetzbarkeitHydrophob
      Physiochemische Informationen
      Porengröße0.45 µm
      Abmessungen
      FilteroberflächeGlatt
      Filterabmessungen26 cm x 26 cm
      Materialinformationen
      Chemie
      • Hydrophobes PVDF
      Angaben zur Toxikologie
      Sicherheitsinformationen gemäß GHS
      Sicherheitshinweise
      Hinweise zum Produktgebrauch
      Lagerungs- und Versandinformationen
      Verpackungsinformationen
      Menge10
      Transportinformationen
      Ergänzende Informationen
      Spezifikationen
      Global Trade Item Number
      Bestellnummer GTIN
      IPVH304F0 04053252471339

      Documentation

      Immobilon-P Membran, PVDF, 0,45 µm, 26 x 26 cm-Blätter SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Literatur

      ÜbersichtAnwendung
      Quantitation of Protein on gels and blots by infrared fluorescence of Coomassie blue and fast green
      Luo S., Wehr N.B., Levine R.L.
      Analytical Biochemistry:350 (2006):233-238  2005

      		Immunoblotting (Western)

      Broschüre

      Titel
      Western Blotting Tools

      Technische Informationen

      Titel
      Protein Blotting Handbook: 6th Edition

      Datenblatt

      Titel
      Immobilon Transfer Membranes: For superior protein and nucleic acid blots

      Gebrauchsanleitungen

      Titel
      Immobilon -P Blotting Sandwiches
      Immobilon®-P Transfer Membrane User Guide