Millipore Sigma Vibrant Logo

371963 H-89, Dihydrochloride - CAS 127243-85-0 - Calbiochem

H-89, Dihydrochloride, CAS 127243-85-0, is a cell-permeable, potent, selective, reversible, ATP-competitive inhibitor of protein kinase A (Ki = 48 nM).

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: N-[2-((p-Bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor III

Primary Target: PKA
View Products on Sigmaaldrich.com
371963
Preis & Verfügbarkeit

Übersicht

Replacement Information

Key Spec Table

CAS #Empirical Formula
127243-85-0C₂₀H₂₀BrN₃O₂S · 2HCl

Preis & Verfügbarkeit

Bestellnummer VerfügbarkeitVerpackung St./Pkg. Preis Menge
371963-1MG
Verfügbarkeit wird abgerufen...
—
Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      		 Kst.-Ampulle 1 mg
      Preis wird abgerufen...
      Preis nicht abrufbar
      Die Mindestmenge muss ein Vielfaches sein von
      Maximum Quantity is
      Bei Bestätigung Weitere Informationen
      Sie haben () gespart
       
      —
      Bitte erfragen
      		 —
      Description
      OverviewA cell-permeable, selective, reversible, ATP-competitive and potent inhibitor of protein kinase A (Ki = 48 nM). Inhibits other kinases only at much higher concentrations: CaM kinase II (Ki = 29.7 µM), casein kinase I (Ki = 38.3 µM), myosin light chain kinase (Ki = 28.3 µM), protein kinase C (Ki = 31.7 µM), and ROCK-II (IC50 = 270 nM). May be used to discriminate between the effects of PKA and cAMP-regulated guanine-nucleotide-exchange factors (GEFs) such as GEFI, GEFII, or Epac (exchange protein directly activated by cAMP). Enhances radiation-induced apoptosis in the human cell line BM 13674. A 10 mM (1 mg/193 µl) solution of H-89, 2HCl (Cat. No. 371962) in DMSO is also available.
      Catalogue Number371963
      Brand Family Calbiochem®
      SynonymsN-[2-((p-Bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor III
      References
      ReferencesLeemhuis, J., et al. 2002. J. Pharmacol. Exp. Ther. 300, 1000.
      Davies, S.P., et al. 2000. Biochem. J. 351, 95.
      Kawasaki, H., et al. 1998. Science 282, 2275.
      de Rooij, J., et al. 1998. Nature 396, 474.
      Findik, D., et al. 1995. J. Cell. Biochem. 57, 12.
      Geilen, C.C. 1992. FEBS Lett. 309, 381.
      Chijiwa, T., et al. 1990. J. Biol. Chem. 265, 5267.
      Combest, W.L., et al. 1988. J. Neurochem. 51, 1581.
      Product Information
      CAS number127243-85-0
      ATP CompetitiveY
      FormClear to off-white crystalline film
      Hill FormulaC₂₀H₂₀BrN₃O₂S · 2HCl
      Chemical formulaC₂₀H₂₀BrN₃O₂S · 2HCl
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      ApplicationH-89, Dihydrochloride, CAS 127243-85-0, is a cell-permeable, potent, selective, reversible, ATP-competitive inhibitor of protein kinase A (Ki = 48 nM).
      Biological Information
      Primary TargetPKA
      Primary Target K<sub>i</sub>48 nM for protein kinase A
      Purity≥99% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for at up to 4 months at 4°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      371963-1MG 04055977213522

      Documentation

      H-89, Dihydrochloride - CAS 127243-85-0 - Calbiochem SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      H-89, Dihydrochloride - CAS 127243-85-0 - Calbiochem Analysenzertifikate

      TitelChargennummer
      371963

      Literatur

      Übersicht
      Leemhuis, J., et al. 2002. J. Pharmacol. Exp. Ther. 300, 1000.
      Davies, S.P., et al. 2000. Biochem. J. 351, 95.
      Kawasaki, H., et al. 1998. Science 282, 2275.
      de Rooij, J., et al. 1998. Nature 396, 474.
      Findik, D., et al. 1995. J. Cell. Biochem. 57, 12.
      Geilen, C.C. 1992. FEBS Lett. 309, 381.
      Chijiwa, T., et al. 1990. J. Biol. Chem. 265, 5267.
      Combest, W.L., et al. 1988. J. Neurochem. 51, 1581.

      Literaturstellen

      Titel
    • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.
      		•
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-April-2011 JSW
      SynonymsN-[2-((p-Bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor III
      DescriptionA cell-permeable, selective, reversible, ATP-competitive and potent inhibitor of protein kinase A (Ki = 48 nM). Inhibits other kinases at several fold higher concentrations: myosin light chain kinase (Ki = 28.3 µM), Ca2+/calmodulin-dependent protein kinase II (Ki = 29.7 µM), protein kinase C (Ki = 31.7 µM), casein kinase I (Ki = 38.3 µM), and Rho Kinase II (IC50 = 270 nM). May be used to discriminate between the effects of PKA and cAMP-regulated guanine-nucleotide-exchange factors (GEFs), such as GEFI or Epac (exchange protein directly activated by cAMP) and GEFII. Reported to induce neurite formation in NG 108-15 cells (~1 mM) by blocking the action of Rho kinase II.
      FormClear to off-white crystalline film
      Intert gas (Yes/No) Packaged under inert gas
      CAS number127243-85-0
      Chemical formulaC₂₀H₂₀BrN₃O₂S · 2HCl
      Structure formulaStructure formula
      Purity≥99% by HPLC
      SolubilityDMSO (200 mg/ml) or H₂O (25 mg/ml)
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for at up to 4 months at 4°C.
      Toxicity Standard Handling
      ReferencesLeemhuis, J., et al. 2002. J. Pharmacol. Exp. Ther. 300, 1000.
      Davies, S.P., et al. 2000. Biochem. J. 351, 95.
      Kawasaki, H., et al. 1998. Science 282, 2275.
      de Rooij, J., et al. 1998. Nature 396, 474.
      Findik, D., et al. 1995. J. Cell. Biochem. 57, 12.
      Geilen, C.C. 1992. FEBS Lett. 309, 381.
      Chijiwa, T., et al. 1990. J. Biol. Chem. 265, 5267.
      Combest, W.L., et al. 1988. J. Neurochem. 51, 1581.
      Citation
    • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.
      		•